人工授精OVUM图像中最佳穿刺位置的检测

Yuya Kinishi, T. Maekawa, S. Mizuta, T. Ishikawa, Y. Hata
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引用次数: 1

摘要

本文旨在通过评价细胞质破裂膜来确定卵子的最佳穿刺位置。我们使用了139个卵子的卵胞浆内单精子注射(Piezo-ICSI)图像。其中,从电影文件(破裂:31,未破裂:108)中获取穿刺前的灰度图像及其实际穿刺位置,并在穿刺位置周围的分析区域计算局部二值模式(Local Binary Pattern, LBP)特征。将LBP特征降维,利用三维特征对数据进行分层聚类分类。因此,将数据分为两组(a和B), a组有7个Ruptures和50个No Ruptures, B组有24个Ruptures和58个No Ruptures。则灵敏度为0.77。因此,从膜的形状特征来评价细胞质破裂膜是可能的。根据特征确定最佳穿刺位置。
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Detection of Optimal Puncture Position in OVUM Images for Artificial Insemination
This paper aims to determine the optimal puncture position of ovum by evaluating rupture membrane of cytoplasm. We employed 139 ovum images on the Piezo-ICSI (Intracytoplasmic sperm injection). In it, grayscale images before puncture and their actual puncture position were obtained from the movie file (Rupture:31, No Rupture:108), and Local Binary Pattern (LBP) feature is calculated at analysis area around the puncture position. LBP feature dimensions are reduced, and data are classified by hierarchical clustering method using feature of three dimensions. As a result, the data classified into two clusters (Clusters A and B). Cluster A has 7 Ruptures and 50 No Ruptures, Cluster B has 24 Ruptures and 58 No Ruptures. Then, the sensitivity is 0.77. Therefore, it is possible to evaluate rupture membrane of cytoplasm from shape feature of membrane. The optimal puncture position could be determined by the features.
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