基于神经内分泌学研究和生物技术创新的鱼群人工繁殖控制系统的发展

Garlov Pavel Evgenyevich, Rybalova Natalia Borisovna, Nechaeva Tamara Alexeyevna, Temirova Saima Umargadzhievna, Turicin Vladimir Sergeevich, Marasayev Sergey Fedorovich
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引用次数: 0

摘要

利用光镜、电镜和免疫细胞化学方法,通过生态组织生理学研究,首次证实了下丘脑-垂体神经分泌系统(HHNS)参与鱼类生殖。在鱼类洄游初期,HHNS的基本渗透调节功能受到了破坏,导致了生境的变化。同时,HHNS以“迁移冲动”的主导状态对中枢神经系统(CNS)觉醒产生嗜神经效应。在产卵初期,HHNS也启动了产卵行为,并通过参与机体对自然生理应激的保护适应性反应来完成产卵行为。HHNS在鱼类繁殖中的功能作用是启动洄游和产卵行为的能量密集型(生殖)过程,并通过抑制靶腺的过度活跃来完成产卵,从而确保身体向节能塑料交换过渡。通过自我调节原则,分析了HHNS在鱼类繁殖整合中的关键作用,从而制定了一个建设性的工作计划。在此基础上,开发了新的生物技术方法来管理育种时间、成活率和珍贵鱼类的生长。它们以10项发明的形式呈现。为了刺激性成熟,开发了分离垂体前叶和垂体后叶的药物并将其引入鲟鱼行业,使其养鱼利用率提高了15%,节省了高达40%的垂体。为了延缓发育期,在海水和食盐溶液的临界盐度为4-8%的生境中,开发了一种工业养鱼保留方法。在此基础上,开发了涵盖养殖生物技术各个阶段的鲟鱼和鲑鱼种群繁殖的完整系统方法。它是由大量繁殖者在海网箱中保留,获得它们的后代,在河流养鱼场的幼鱼生长到它们准备迁徙后,最后在海网箱花园中培养它们,直到大的具有生命抗性的幼鱼。为了实施拟议的工业生物技术和全年养鱼,利用鱼类养殖生境的淡季地下调节和自然-工业工程生态学原理,开发了循环水产养殖系统(RAS)孵化场和养殖场的新装置。由于提高生物技术的繁殖效率和逻辑关系的总目标,所有的发展都被纳入鱼类种群繁殖的生物技术管理系统。
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Development of the control system for Artificial Reproduction of Fish Populations Based on a Complex of Neroendocrinological research and Biotech Innovations
The participation of the hypothalamic-hypophysial neurosecretory system (HHNS) in fish reproduction using light, electron microscopy and immunocytochemistry was established by ecologo-histophysiological studies for the first time in the world literature. At the beginning of passing fish migrations a violation of the HHNS basic osmoregulative function, causing habitat change is revealed. Simultaneously the HHNS causes the neurotropic effect on the central nervous system (CNS) arousal in the form of a dominant state of "Migration impulse." At the beginning of spawning, HHNS also initiates spawning behavior and completes it by participating in the body's protective-adaptive reactions to natural physiological stress. The functional role of the HHNS in fish reproduction is to initiate energy-intensive (reproductive) processes of migratory and spawning behaviors and to complete spawning by suppressing the hyperactivity of target glands, which ensures the body's transition to energy-saving plastic exchange. The analysis of the key role of the HHNS in the integration of fish reproduction by the self-regulation principle has allowed the development of a constructive working scheme. New biotech methods of managing breeding time, rates of survival and growth of valuable fish species were developed on this basis. They are presented in the form of 10 inventions. To stimulate sex maturation, drugs of isolated anterior and posterior pituitary lobes were developed and introduced in sturgeon industry, increasing the extent of their fish-breeding use by 15% and saving the pituitary up to 40%. To delay puberty, a method of industrial fish breeders reservation in the critical salinity habitat of 4-8%, both in seawater and in table salt solutions has been developed. On this basis, a full-system method of sturgeon and salmon fish populations reproduction, covering all stages of farm biotechnology has been developed. It is carried out by mass breeders reservation in sea cages, obtaining there offspring and, after the river fish-farm growing youngs to their readiness for migration, the final cultivation them in sea cages gardens till large life-resistant youngs. For the implementation of the proposed industrial biotechnology and year-round fish farming a new installation of recirculating aquaculture systems (RAS) hatcheries and farms was developed by means of the off-season underground conditioning of fish cultivation habitat and by the principle of natural-industrial engineering ecology. Due to the general aim ‒ increasing the efficiency biotechnology of reproduction and logical relationships, all developments are integrated into the system of biotech management of fish population reproduction.
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