Study of the multi-binding biotinylated Iron oxide nanoparticles (biotin-IONPs) as a promising versatile label material for magnetic biodetection devices

By realizing the multi-binding of magnetic labels with each biomolecule, the sensitivity of magnetic biodetection devices can be improved. In this study, biotinylated iron oxide nanoparticles (biotin-IONPs) were synthesized, and their multi-binding with streptavidin-functionalized biomolecules was investigated. The final biotin-IONPs with 17 nm magnetic core size exhibit superparamagnetic behavior, hydrophilic stability, and multi-binding ability with streptavidin-functionalized antibodies both on gold surfaces and suspended in solution. Consequently, biotin-IONPs are promising magnetic label materials for the improvement of magnetic biodetection devices.