Standardized Criterion of Presepsin Reference Interval for Accurate and Early Diagnosis of Sepsis

Presepsin is a soluble analog of CD14, recently validated as a new biomarker for sepsis. Different cut-off levels of Presepsin for the diagnosis of sepsis have been proposed however, the optimum threshold for early clinical discrimination of sepsis from non-infectious conditions remains unknown. Presepsin is a soluble analog of CD14, recently validated as a new biomarker for sepsis. Different cut-off levels of Presepsin for the diagnosis of sepsis have been proposed, however the optimum threshold for early clinical discrimination of sepsis from non-infectious conditions remained unknown. This work was undertaken to define the reference interval of Presepsin for Sepsis according to the CLSI C28-A3c approved guideline. This study evaluated the reference interval of Presepsin for early diagnosis and differentiation of Sepsis. Reference individuals (N=72) receiving in-patient care at ICU for sepsis and complications were selected for the study. Presepsin concentrations were measured by enzyme-linked immunosorbent assay. Reference limits were calculated using the non-parametric robust method. Reference individuals (N=72) receiving in-patient care at ICU for sepsis and complications were selected for the study. Presepsin concentrations were measured by enzyme linked immunosorbent assay. Reference limits were calculated using the non-parametric robust method. Overall, the reference range for Presepsin in sepsis patients was 24.2 -872.2 pg/mL (90% confidence interval). Peak values for males were relatively higher than for females and the presepsin concentrations were influenced by age. Overall, the reference range for the Presepsin in sepsis patients was 24.2 -872.2 pg/mL (90% confidence interval). Peak values for males were relatively higher than females and the presepsin concentrations were influenced by age. Proposed reference interval could assist in accurate and early diagnosis of sepsis which would eventually improve the outcome in patients of all age groups. Proposed reference interval could assist in accurate and early diagnosis of sepsis which would eventually improve outcome in patients of all age groups