{"title":"土壤微生物的脱氧核糖核酸碱基组成及核苷酸分布。","authors":"Rainer Gebers , Manley Mandel , Peter Hirsch","doi":"10.1016/S0721-9571(81)80026-9","DOIUrl":null,"url":null,"abstract":"<div><p>The DNAs of seven bacterial strains belonging to <em>Pedomicrobium ferrugineum</em> and <em>P. manganicum</em> were investigated by buoyant density centrifugation and thermal denaturation experiments. The DNA base composition was calculated to range from 66.3 to 67.3% GC in CsCl gradients and from 64.8 to 65.7% GC in thermal transition profiles. The correlation of both methods was T<sub>mo.issc</sub> = 428.5 (ϱ — 1.533) under our experimental conditions. Analysis of the DNA nucleotide distributions revealed a homogeneous group characterized by Gaussian distribution with a mean width of 9.19 ± 0.14% GC (uncorrected) and an average degree of asymmetry of 1.16 ± 0.04.</p></div>","PeriodicalId":101290,"journal":{"name":"Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","volume":"2 4","pages":"Pages 332-338"},"PeriodicalIF":0.0000,"publicationDate":"1981-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0721-9571(81)80026-9","citationCount":"13","resultStr":"{\"title\":\"Deoxyribonucleic Acid Base Composition and Nucleotide Distribution of Pedomicrobium spp.\",\"authors\":\"Rainer Gebers , Manley Mandel , Peter Hirsch\",\"doi\":\"10.1016/S0721-9571(81)80026-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The DNAs of seven bacterial strains belonging to <em>Pedomicrobium ferrugineum</em> and <em>P. manganicum</em> were investigated by buoyant density centrifugation and thermal denaturation experiments. The DNA base composition was calculated to range from 66.3 to 67.3% GC in CsCl gradients and from 64.8 to 65.7% GC in thermal transition profiles. The correlation of both methods was T<sub>mo.issc</sub> = 428.5 (ϱ — 1.533) under our experimental conditions. Analysis of the DNA nucleotide distributions revealed a homogeneous group characterized by Gaussian distribution with a mean width of 9.19 ± 0.14% GC (uncorrected) and an average degree of asymmetry of 1.16 ± 0.04.</p></div>\",\"PeriodicalId\":101290,\"journal\":{\"name\":\"Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie\",\"volume\":\"2 4\",\"pages\":\"Pages 332-338\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1981-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0721-9571(81)80026-9\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0721957181800269\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0721957181800269","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Deoxyribonucleic Acid Base Composition and Nucleotide Distribution of Pedomicrobium spp.
The DNAs of seven bacterial strains belonging to Pedomicrobium ferrugineum and P. manganicum were investigated by buoyant density centrifugation and thermal denaturation experiments. The DNA base composition was calculated to range from 66.3 to 67.3% GC in CsCl gradients and from 64.8 to 65.7% GC in thermal transition profiles. The correlation of both methods was Tmo.issc = 428.5 (ϱ — 1.533) under our experimental conditions. Analysis of the DNA nucleotide distributions revealed a homogeneous group characterized by Gaussian distribution with a mean width of 9.19 ± 0.14% GC (uncorrected) and an average degree of asymmetry of 1.16 ± 0.04.
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