在某些食品工业副产品上生长的青霉sp 7/4B/EI 1突变体的聚半乳糖醛酸酶活性

I Szajer
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摘要

短句来源研究了有效合成PG而不能产生PE的青霉sp 7/4B/E11突变体对食品工业副产物(苹果浆、甜菜浆、苹果浆或甜菜浆与麦麸的混合物)的溶胃活性,并在部分实验中添加了Czapek培养基的无机盐。菌株在不含矿物盐的副产物(主要是甜菜粕)上生长时,PG活性较低,但生长良好;在含矿物盐的副产物(主要是甜菜粕)上培养后,PG活性有所提高。矿物盐在甜菜果肉(3%)上培养6 ~ 8 d后,PG活性最高。在所测盐中,NaNO3对PG活性的影响最大,K2HPO4和MgSO4的影响较小,而其他盐对PG活性的影响较小。在这些培养条件下,突变体没有合成PE。
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Polygalacturonase activity of Penicillum sp 7/4B/EI 1 mutant grown on some by-products of food industry.

Pectolytic activity of the Penicillum sp 7/4B/E11 mutant, which synthesized effectively PG and was not capable of producing PE, was studied on some by-products of food industries (apple pulp, beet pulp, and mixtures of apple pulp or beet pulp with wheat bran) supplemented in some experiments with mineral salts of the Czapek's medium. The strain showed good growth but a low PG activity when grown on by-products without mineral salts and an increase in PG activity after cultivation on these by-products (mainly on beet pulp) enriched with mineral salts. The highest PG activity was obtained after 6-8 days cultivation on beet pulp (3%) with mineral salts. Among the tested salts, NaNO3 influenced PG activity most effectively, K2HPO4 and MgSO4 to a smaller degree whereas others were ineffective. The mutant did not synthesize PE under these culture conditions.

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