将APEX标记与单个细胞器成像质谱相结合，揭示了溶酶体蛋白周转的异质性

The Journal of Cell Biology Pub Date : 2019-11-12 DOI:10.1083/jcb.201901097

Derek P. Narendra, C. Guillermier, F. Gyngard, Xiaoping Huang, Michael E. Ward, M. Steinhauser

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引用次数: 16

摘要

纳伦德拉等人证明APEX可以作为同位素比值成像质谱的第一个遗传编码报告基因。在单个溶酶体中测量了大量蛋白质周转，显示了单个细胞内溶酶体之间意想不到的异质性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Coupling APEX labeling to imaging mass spectrometry of single organelles reveals heterogeneity in lysosomal protein turnover

Narendra et al. demonstrate that APEX can be used as the first genetically encoded reporter for isotope ratio imaging mass spectrometry. Bulk protein turnover is measured in individual lysosomes demonstrating unanticipated heterogeneity among lysosomes within a single cell.

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The Journal of Cell Biology

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