开伯尔-普赫图赫瓦省首例马轮状病毒感染报告

A. Durrani, M. Husnain, Ikramul Haq, Ulfat Batool, Fahimullah Khan
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摘要

轮状病毒是马腹泻的主要原因。6个月大的马驹主要受影响。本研究是在KPK地区白沙瓦、查尔萨达和马尔丹进行的。本研究选取3个地区共600只动物,观察马的阳性率。从受感染的马身上收集粪便样本,保存在福尔马林中并送到实验室。同时采集血样作血液学检查和血清化学检查。采用市售的酶联免疫吸附试验(ELISA)对腹泻马驹进行轮状病毒检测。结果表明,白沙瓦区轮状病毒患病率为11.5%,查尔萨达区为14%,马尔丹区为18%。轮状病毒感染率女性为17.11%,男性为12.46%。驴马驹轮状病毒感染率为16.32%,马马驹轮状病毒感染率为12.06%。对腹泻马驹1 ~ 6月龄的血液学指标和血清学指标进行了观察。结果表明:腹泻马驹1 ~ 6月龄血液学指标rbc、PCV和中性粒细胞升高,1 ~ 6月龄血红蛋白、淋巴细胞、嗜碱性粒细胞和单核细胞降低。所有年龄组的白细胞和嗜酸性粒细胞值保持正常。测定腹泻马驹的血清学指标、血清酶和血清电解质水平。结果表明:1 ~ 6月龄各组肌酐、胆红素、白蛋白、尿素氮、ALT、AST、GGT和钙离子值升高,葡萄糖、ALP、钠、钾和氯离子值降低;本研究有助于马腹泻病因的诊断和对腹泻马驹的治疗。观察腹泻马驹的血液学、血清化学和血清电解质值也有一定的参考价值。
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First Report of Equine Rotavirus Infection in Khyber Pakhtunkhwa
Rotavirus is the main cause of diarrhea in equines. Young foals at 6 month of age are mainly affected. The present study was carried out in KPK districts Peshawar, Charsada and Mardan. In this study total 600 animals were selected to observe the prevalence of positive percentage in equines in three districts. Fecal sample were collected from affected equines preserved in formalin and send to laboratory. Blood samples also collected for hematological examination and serum chemistry. For presence of rotavirus foal suffering from diarrhea were screened and examined by using commercially available ELISA kit. The results show that the rotavirus prevalence was 11.5% in district Peshawar, 14% in district Charsada and 18% in District Mardan. The rotavirus prevalence was 17.11% in female and 12.46% in males. The rotavirus prevalence was 16.32% in donkey foals 12.06% in horse foals. Hematological values and serological values of diarrheic foal are also observed in from 1st Month to 6th Month of age group. The results shows that hematological values like RBCs, PCV and Neutrophil values are increased from1st Month to 6th Month of age group of diarrheic foal and Hemoglobin, Lymphocytes, Basophil and Monocytes values are decreased in age group from 1stMonth to 6th month of age. WBCs and Eosinophil values remain normal in all age groups. Whereas the serological values, serum enzymes level and serum electrolyte level of the diarrheic foals were observed. The results shows that Creatinine, Bilirubin, Albumin, Urea Nitrogen, ALT, AST, GGT and Calcium ion values increased whereas Glucose, ALP, Sodium, Potassium and Chloride values decreased in age group of 1st Month to 6th Month. The present study is helpful to diagnose the cause of diarrhea in equines and helpful to treat the diarrheic foal.It is also helpful to observe the hematological, serum chemistry and serum electrolytes values in diarrheic foals.
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