生物合成银纳米颗粒乙醇提取物(Moringa oleifera),以抗氧化剂的形式测试其活性

Fujiana Abd Karim, Robert Tungadi, Nur Ain Thomas
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引用次数: 3

摘要

辣木叶可能具有很高的抗氧化剂,因为它含有次级代谢物,其中之一是槲皮素。因此,为了避免在合成纳米银时使用有害化学物质,它是用辣木叶提取物的生物还原剂制成的。本研究旨在通过DPPH方法了解银纳米颗粒形成的最佳温度,对纳米颗粒进行表征,并对辣木叶抗氧化AgNPs进行研究。这是一项实验室实验研究。纳米颗粒的合成使用浓度为0.4%的辣木叶提取物,将其与浓度为1 mM的AgNO3按1:9的比例在60oC, 70oC和80oC的不同温度下反应30分钟。采用紫外-可见分光光度法对纳米颗粒进行了表征,发现纳米颗粒形成的最佳温度为80℃。然后继续使用PSA进行表征,并显示纳米颗粒在80℃温度下的平均尺寸,即82,9 nm, PDI值为0,225。计算结果表明,辣木叶的IC50 AgNPs值为61.78 ppm,属于强类,而未添加纳米银颗粒的辣木叶粗提物的IC50 AgNPs值为124.41 ppm,属于弱类。
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BIOSINTESIS NANOPARTIKEL PERAK EKSTRAK ETANOL 96% DAUN KELOR (Moringa oleifera) DAN UJI AKTIVITASNYA SEBAGAI ANTIOKSIDAN
Moringa leaf potentially has a high antioxidant becouse it contains secondary metabolite, one of which is quercetin. Therefore, to avoid harmful chemicals in the synthesis of silver nanoparticles, it is made by using a bio reductant of Moringa leaf extract. This study aims to know the optimal temperature in the formation of silver nanoparticles, to characterize the nanoparticle, and to rest the antioxidant AgNPs of Moringa leaf by using the DPPH method. This is a laboratory experimental study. The synthesis of nanoparticles uses a concentration of 0,4% Moringa leaf extract, which is reacted to AgNO3 with a concentration of 1 mM in a ratio of 1:9 at various temperatures of 60oC, 70oC, and 80oC for 30 minutes. The formed nanoparticles are characterized by using UV-VIS spectrophotometry and showing the optimum temperature for nanoparticles formation, which is 80oC. It is then continued to characterize using PSA and showing the average size of nanoparticles at a temperature of 80oC, which is 82,9 nm with a PDI value of 0,225. The result of the calculation of IC50 AgNPs shows that Moringa leaf obtains a value of 61.78 ppm, which is included in the strong category, meanwhile the thick extract of Moringa leaf without the addition of silver nanoparticles obtain a value of 124.41 ppm, which is included in the weak category.
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