用于啮齿类动物的低成本,61通道微ECoG阵列

V. Woods, Charles Wang, S. Bossi, Michele N. Insanally, M. Trumpis, R. Froemke, J. Viventi
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引用次数: 9

摘要

微皮质电图(μECoG)提供了一个具有大面积皮质的微创、高分辨率界面。各种各样的μECoG设计已经开发和定制[1]-[4],包括有源,多路复用阵列[5]和溶解基板上的阵列,以增加保形接触[6]。然而,设计和制造定制的μECoG阵列需要使用微加工设备,这是许多神经科学实验室所没有的。微加工也是典型的劳动密集型和昂贵的。商业μECoG阵列具有64个电极和更粗的尺寸,成本约为1000美元,限制了它们对大量动物慢性植入的适用性。在这里,我们提出了一种高密度(406 μm间距),柔性(~30 μm薄),61触点μECoG电极阵列,采用低成本的商业制造工艺制造。当订购数量为100个时,该阵列的成本仅为26美元,当订购数量较少时,每个电极的成本略有增加。细间距导线使连接的尺寸最小化,使啮齿类动物能够长期植入。制备的μECoG阵列的内部后处理添加了可选的电极涂层,如铂黑,以降低电极阻抗。我们的电极设计和制造工艺大大提高了高容量,高分辨率神经科学的可及性并降低了成本。
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A low-cost, 61-channel µECoG array for use in rodents
Micro-Electrocorticography (μECoG) offers a minimally invasive, high resolution interface with large areas of cortex. A wide variety of μECoG designs have been developed and customized [1]-[4], including active, multiplexed arrays [5] and arrays on dissolving substrates for increased conformal contact [6]. However, designing and fabricating customized μECoG arrays requires access to microfabrication facilities, which many neuroscience labs do not have. Microfabrication is also typically labor intensive and expensive. Commercial μECoG arrays with 64 electrodes and coarser dimensions cost approximately $1000, limiting their suitability for chronic implantation in large numbers of animals. Here we present a high density (406 μm spacing), flexible (~30 μm thin), 61-contact μECoG electrode array fabricated using a low-cost, commercial manufacturing process. The array costs just $26 when ordered in quantities of 100, with the cost per electrode increasing slightly when lower quantities are ordered. Fine pitch wires minimize the size of the interconnections, enabling chronic implantation in rodents. In-house post-processing of the fabricated μECoG arrays added optional electrode coatings, such as platinum black, to reduce the electrode impedance. Our electrode design and manufacturing process dramatically improves the accessibility and reduces the cost of high-volume, high-resolution neuroscience.
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