用内洛尔公牛的性精液进行体外胚胎生产

Eduarda Letícia da Silva Barrozo, V. Nascimento, Marcia Dias
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摘要

目的是分析精液类型,常规和性别,对体外胚胎生产的影响。与常规精液受精成熟的卵母细胞1,285个,与有性精子受精的卵母细胞1,155个,分别获得1,167个(90.55%)和1,051个(90.68%)受精卵(P=0.8411)。在离体培养中,来自常规精液的受精卵有769个(67.42%)进行了胚胎分裂,来自有性精子的受精卵有540个(55.12%)进行了胚胎分裂(P=0.8411)。两种分析变量均无统计学差异。在第7天,评估产生的活胚数:常规胚428个(53.55%),性精胚138个(30.64%)(P=0.0001)。因此,相对于内洛雷公牛的有性精子,它与常规精液的胚胎转化效果更好。
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Produção de embriões in vitro com sêmen sexado de touros nelore
The objective was to analyze the effect of the type of semen, conventional and sexed, on the in vitro production of embryos. A total of 1,285 oocytes and 1,155 oocytes with sexed semen were matured and fertilized with conventional semen, obtaining 1,167 (90.55%) and 1,051 (90.68%) zygotes in fertilization with conventional and sexed semen, respectively (P=0.8411). In in vitro culture, embryo cleavage was evaluated in 769 (67.42%) of zygotes from conventional semen and 540 (55.12%) from zygotes from sexed semen (P=0.8411). There was no statistical difference in both analyzed variables. On day 7, the number of viable embryos produced was evaluated, being 428 (53.55%) conventional embryos and 138 (30.64%) viable embryos from sexed semen (P=0.0001). There was a significant difference, therefore, it obtained better embryonic conversion with conventional semen in relation to sexed semen of Nelore bulls.
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