新型逆转录-聚合酶链反应(RT-PCR)检测装置的研究

Russelle Go, Lee Romasanta, Pamela Faye Daza, Darel Alexie David, Luieza Sabello, M. N. Young
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摘要

2019年底,新型冠状病毒病(COVID-19)爆发,至今仍是当今世界的头号问题。这种病毒可以通过感染者打喷嚏、咳嗽和呼气时的唾液飞沫传播。到目前为止,总共有1.11亿例病毒病例,并引入了一些技术来帮助发现感染并减少病毒的传播。其中一项技术是逆转录聚合酶链反应(RT-PCR)机。这台机器可以检测任何特定的遗传物质,包括病毒。来自人体的样本用几种化学溶液处理,这些化学溶液可以去除物质,只允许核糖核酸(RNA)保留。但这台机器的问题是产生假阳性和假阴性结果以及某些故障。由于使用RT-PCR存在问题,我们的团队提出了一种更新的临时版本,并将其命名为covid - bit。covid - bit是一种更简化和便携的RT-PCR版本,价格更便宜。在这项研究中,团队使用SEM模型对covid - bit作为病毒检测设备和RT-PCR机的替代品进行了分析,发现在使用的有效性和便携性方面存在显著差异,并显示来自医疗行业的210名受访者最有可能使用这类机器。
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A Study on COVIDBIT as a COVID-19 Detection Device for an Alternative Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Machine
At the end of 2019, Coronavirus disease (COVID-19) started and as of now, it is still the number one problem of the world today. This virus can be transmitted through droplets of saliva from an infected person which can be from a sneeze, cough, and exhales. As of now, there are a total of 111 million cases of the virus and there are technologies that were introduced to help in the detection of the infection and to reduce the spread of the virus. One of this technology is the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Machine. This machine detects any specific genetic material, including a virus. Samples from the body are treated with several chemical solutions which remove substances and only allow Ribonucleic Acid (RNA) to remain. But the problem with this machine is the elicitation of false-positive and false-negative results and certain malfunctions. Due to the issues in using RT-PCR, our team has come up with a newer and improvised version of the machine and called it COVIDBIT. COVIDBIT is a more simplified and portable version of the RT-PCR at a cheaper price. In this study, the team analyzed COVIDBIT as a virus detection device and an alternative for RT-PCR machine using SEM model and found out that there is a significant difference in terms of effectiveness and portability in usage and showed that the 210 respondents from the medical industry are most likely to use these kinds of machine.
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