光谱分析法测定红细胞形态的计量特性评价

Y. Sokol, K. Kolisnyk, T. V. Bernads’ka
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摘要

光谱光度法在医学生物学中广泛应用于生物分子的定量和定性分析。这种方法是基于分子吸收电磁辐射的能力。现代临床实验室诊断广泛使用依赖于这些半透明物体(如血液成分)的物理特性的光学分析方法。了解血液及其成分的吸收光谱,就可以通过求解与这些光谱相对应的数学方程组来量化所有成分的浓度。然而,现有的红细胞光学分析方法不允许量化其几何参数,这些几何参数也可能指示某些诊断征象,并用于分析患者身体的临床状况。这项工作的目的是评估新开发的方法,确定红细胞的几何参数,它结合了光谱分析和双重退火的计量特性。红细胞三维成像的输入数据取自样品在自然光和不同波长的相干光源照射下的图像。后者,在一些额外的图像相关之后,增加了结果的可靠性。对数字干涉显微镜的误差和测量通道分辨率的计算结果表明,该方法具有可接受的精度。该方法获得的三维图像精度比其他已知方法提高20%以上。这可以更准确地确定红细胞结构的信息几何参数,并利用它们获得患者身体的额外临床诊断特征。
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Evaluation of metrological characteristics of spectral analysis method for determining erythrocyte morphology
Spectral photometry is currently widely used for quantitative and qualitative analysis of biological molecules in medical biology. The method is based on the ability of molecules to absorb electromagnetic radiation. Modern clinical laboratory diagnostics extensively uses optical methods of analysis that rely on these physical properties of semitransparent objects, such as blood components. Knowing the absorption spectra of blood and its components, it is possible to quantify the concentration of all the components by solving the mathematical system of equations corresponding to these spectra. However, the existing methods of optical analysis of erythrocytes do not allow quantifying their geometric parameters, which may also indicate certain diagnostic signs and be used to analyze the clinical condition of the patient's body. The aim of this work is to evaluate the metrological characteristics of the newly developed method of determining the geometric parameters of erythrocytes, which combines spectral analysis and double annealing. The input data for the 3D imaging of erythrocytes were taken from the images of the sample both made in natural light and illuminated by a coherent light source with different wavelengths. The latter, after some additional image correlation, increases the reliability of the result. The calculation results on the errors and the measuring channel resolution of the digital interference microscope indicate an acceptable accuracy of the method. The accuracy of the three-dimensional image obtained by the proposed method is more than 20% higher than that of other known methods. This allows determining the informative geometric parameters of the structure of erythrocytes more accurately and using them to obtain additional clinical diagnostic characteristics of the patient's body.
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