S. Ghodbane, A. Lahbib, S. Ennaceur, M. Sakly, M. R. Driss
{"title":"一种测定大鼠血浆中生育酚的高效液相色谱新方法","authors":"S. Ghodbane, A. Lahbib, S. Ennaceur, M. Sakly, M. R. Driss","doi":"10.1051/ATA/2013033","DOIUrl":null,"url":null,"abstract":"Objective: The objective of the present work is the simplification of sample treatment for vitamin E measurements in rat plasma by high-performance liquid chromatography (HPLC), then the evaluation of the effect of cadmium treatment on the vitamin E level. Methods: A HPLC method to determine vitamin E was developed with direct extraction with n-hexane-dichloromethane. The dry residue was redissolved in methanol. The method employs a Supelco Discovery ® C18 column and methanol/water (95:5, v/v) as the mobile phase. After being developed, the method was validated with a fluorescence detector. To examine the effects of exogenous cadmium on the plasma vitamin E concentrations, male Wistar rats (weighing 150 ± 20 g) were exposed for 48 h and 10 days to oral intake of 15 and 30 mg/L cadmium (as CdCl 2 ) and vitamin E (10 mg/Kg of diet) simultaneously. Results: This method makes sample treatment easier, especially when working with a large number of samples. It has proved to be selective, linear, accurate and precise. Intoxication with cadmium was followed by a significant decrease in plasma vitamin E concentrations. The lowest level was obtained in the 30 mg/L dosing group after 10 days of cadmium administration. Conclusion: We describe a rapid reversed-phase (RP) HPLC procedure for the determination of vitamin E in plasma. This method might be useful in routine assessment because it saves on solvents and chromatographic time. Interestingly, α -tocopherol may play an important role in preventing oxidative stress induced by cadmium exposure by scavenging free radicals.","PeriodicalId":117929,"journal":{"name":"Annales De Toxicologie Analytique","volume":"2015 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"A new HPLC method for the determination of tocopherol in rat plasma\",\"authors\":\"S. Ghodbane, A. Lahbib, S. Ennaceur, M. Sakly, M. R. Driss\",\"doi\":\"10.1051/ATA/2013033\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: The objective of the present work is the simplification of sample treatment for vitamin E measurements in rat plasma by high-performance liquid chromatography (HPLC), then the evaluation of the effect of cadmium treatment on the vitamin E level. Methods: A HPLC method to determine vitamin E was developed with direct extraction with n-hexane-dichloromethane. The dry residue was redissolved in methanol. The method employs a Supelco Discovery ® C18 column and methanol/water (95:5, v/v) as the mobile phase. After being developed, the method was validated with a fluorescence detector. To examine the effects of exogenous cadmium on the plasma vitamin E concentrations, male Wistar rats (weighing 150 ± 20 g) were exposed for 48 h and 10 days to oral intake of 15 and 30 mg/L cadmium (as CdCl 2 ) and vitamin E (10 mg/Kg of diet) simultaneously. Results: This method makes sample treatment easier, especially when working with a large number of samples. It has proved to be selective, linear, accurate and precise. Intoxication with cadmium was followed by a significant decrease in plasma vitamin E concentrations. The lowest level was obtained in the 30 mg/L dosing group after 10 days of cadmium administration. Conclusion: We describe a rapid reversed-phase (RP) HPLC procedure for the determination of vitamin E in plasma. This method might be useful in routine assessment because it saves on solvents and chromatographic time. Interestingly, α -tocopherol may play an important role in preventing oxidative stress induced by cadmium exposure by scavenging free radicals.\",\"PeriodicalId\":117929,\"journal\":{\"name\":\"Annales De Toxicologie Analytique\",\"volume\":\"2015 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annales De Toxicologie Analytique\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1051/ATA/2013033\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annales De Toxicologie Analytique","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1051/ATA/2013033","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A new HPLC method for the determination of tocopherol in rat plasma
Objective: The objective of the present work is the simplification of sample treatment for vitamin E measurements in rat plasma by high-performance liquid chromatography (HPLC), then the evaluation of the effect of cadmium treatment on the vitamin E level. Methods: A HPLC method to determine vitamin E was developed with direct extraction with n-hexane-dichloromethane. The dry residue was redissolved in methanol. The method employs a Supelco Discovery ® C18 column and methanol/water (95:5, v/v) as the mobile phase. After being developed, the method was validated with a fluorescence detector. To examine the effects of exogenous cadmium on the plasma vitamin E concentrations, male Wistar rats (weighing 150 ± 20 g) were exposed for 48 h and 10 days to oral intake of 15 and 30 mg/L cadmium (as CdCl 2 ) and vitamin E (10 mg/Kg of diet) simultaneously. Results: This method makes sample treatment easier, especially when working with a large number of samples. It has proved to be selective, linear, accurate and precise. Intoxication with cadmium was followed by a significant decrease in plasma vitamin E concentrations. The lowest level was obtained in the 30 mg/L dosing group after 10 days of cadmium administration. Conclusion: We describe a rapid reversed-phase (RP) HPLC procedure for the determination of vitamin E in plasma. This method might be useful in routine assessment because it saves on solvents and chromatographic time. Interestingly, α -tocopherol may play an important role in preventing oxidative stress induced by cadmium exposure by scavenging free radicals.