Assessment of Anti-seizure Property of Plumbago indica L. on Zebrafish Model

The purpose of the current experiment was to determine the anti-convulsant activity of methanolic extract of Plumbago indica L.  In a 50 ml beaker 200 mg of the methanolic extract of Plumbago indica L. was dissolved in water containing the experimental zebrafish. After about 20 minutes the fish was administered with pentylenetetrazole (PTZ) (30 mg in 0.5 ml water) intraperitoneally (i.p) to induce seizure in it. The experimental zebrafish immersed in methanolic extract of Plumbago indica L. and administered with PTZ i.p showed clonic seizure-like activity within a few seconds of administering the PTZ. After about 15 minutes, the experimental zebrafish was found to swim normally i.e., short swim at the top of the tank with no seizure activity. The behavioral profile during 15 minutes of immersion into methanolic extract of Plumbago indica L. and then intraperitoneum (i.p) administration of PTZ was characterized by stages defined as scores: (0) short swim (top of the tank), (1) increased swimming activity, (2) burst swimming (left and right movements), (3) circular movements, (4) clonic seizure-like behavior, (5) fall to the bottom of the tank and tonic seizure-like behavior, (6) death. To attain all scores, zebrafish was exposed to anti-convulsants and administered with PTZ showed varying seizure profiles, severity, and latencies. The study's findings revealed that Plumbago indica L. has significant pharmacological properties. Methanolic extract of Plumbago indica L. has a notable CNS depressive and anti-convulsant effect.