[人类和动物病毒膜蛋白的鉴定]。

A V Ustin, A A Kolontsov, V V Makarov
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引用次数: 0

摘要

综述了病毒粒子和感染细胞表面病毒特异性蛋白的鉴定方法。讨论了膜蛋白的同位素标记、蛋白水解酶的提取和洗涤剂的选择性溶解。介绍了用离心分离法和微载体分离法分离质膜的方法。提出了单克隆抗体和放射免疫沉淀法定位膜蛋白的方法。
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[The identification of the membrane proteins of human and animal viruses].

The review deals with the methods of identification of virus-specific proteins on virion and infected cell surface. The isotopic labeling of membrane proteins, their extraction by proteolytic enzymes and selective solubilization by detergents are considered. The plasmatic membrane isolation by centrifugation and by means of microcarriers is described. The methods of membrane protein localization using monoclonal antibodies and radioimmunoprecipitation are presented.

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