通过克罗能分解细菌分解渗透酸

Uwe Tittmann, Willi Wegst, Renate Blecher, Franz Lingens
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引用次数: 5

摘要

氯唑酮降解菌E与反式肉桂酸共同培养,培养基中积累了顺式-2.3-二氢-2.3-二羟基反式肉桂酸。氯唑酮降解菌N与反式肉桂酸孵育,在培养基中积累了2.3-二羟基反式肉桂酸。每个代谢物都以结晶形式分离出来,并通过各种常规化学技术进行鉴定-从菌株E制备的儿茶酚-2.3-双加氧酶将2.3-二羟基反式肉桂酸转化为中间裂解产物。细胞提取物转化黄环裂变产物。采用高效液相色谱法和富马酸酶法检测富马酸的生成。
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Abbau von trans-Zimtsäure durch Chloridazon-abbauende Bakterien

A chloridazone-degrading bacterium strain E incubated with trans-cinnamic acid accumulated cis-2.3-dihydro-2.3-dihydroxy-trans-cinnamic acid in the culture medium. A chloridazone-degrading bacterium strain N incubated with trans-cinnamic acid, accumulated 2.3-dihydroxy-trans-cinnamic acid in the culture medium. Each metabolite was isolated in crystalline form and identified by a variety of conventional chemical techniques-Catechol-2.3-dioxygenase prepared from the strain E converted 2.3-dihydroxy-trans-cinnamic acid to the meta cleavage product. Cell extracts converted the yellow ring fission product. The production of fumaric acid could be detected with high performance liquid chromatography and with fumarase.

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