{"title":"基于氨苄西林诱导的大肠杆菌营养不良菌裂解的微生物测定","authors":"G. Krapf, W. Bode","doi":"10.1016/S0172-5564(80)80025-X","DOIUrl":null,"url":null,"abstract":"<div><p>A novel quantitative microbiological assay is presented. This is based on the finding that the rate of ampicillin-induced lysis of cultures of an auxotrophic <em>E. coli</em> mutant depends on the concentration of the required growth factor the mutant is defective for.</p><p>Contrary to the usual methods the new procedure is less time consuming and needs no more than 60 to 90 minutes, there is no requirement for sterile conditions. It was possible to estimate amounts of about 0.5 <em>μ</em>g/ml in raw extracts of biological material.</p></div>","PeriodicalId":101294,"journal":{"name":"Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","volume":"1 4","pages":"Pages 314-319"},"PeriodicalIF":0.0000,"publicationDate":"1980-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5564(80)80025-X","citationCount":"2","resultStr":"{\"title\":\"A microbiological assay based on ampicillin-induced lysis of Escherichia coli auxotrophs\",\"authors\":\"G. Krapf, W. Bode\",\"doi\":\"10.1016/S0172-5564(80)80025-X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A novel quantitative microbiological assay is presented. This is based on the finding that the rate of ampicillin-induced lysis of cultures of an auxotrophic <em>E. coli</em> mutant depends on the concentration of the required growth factor the mutant is defective for.</p><p>Contrary to the usual methods the new procedure is less time consuming and needs no more than 60 to 90 minutes, there is no requirement for sterile conditions. It was possible to estimate amounts of about 0.5 <em>μ</em>g/ml in raw extracts of biological material.</p></div>\",\"PeriodicalId\":101294,\"journal\":{\"name\":\"Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie\",\"volume\":\"1 4\",\"pages\":\"Pages 314-319\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1980-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0172-5564(80)80025-X\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S017255648080025X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S017255648080025X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A microbiological assay based on ampicillin-induced lysis of Escherichia coli auxotrophs
A novel quantitative microbiological assay is presented. This is based on the finding that the rate of ampicillin-induced lysis of cultures of an auxotrophic E. coli mutant depends on the concentration of the required growth factor the mutant is defective for.
Contrary to the usual methods the new procedure is less time consuming and needs no more than 60 to 90 minutes, there is no requirement for sterile conditions. It was possible to estimate amounts of about 0.5 μg/ml in raw extracts of biological material.
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