龙血树植物微繁的研究

Gehan M. Y. Salama, A. S. Tawila, Abla H. Dergham
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摘要

本实验旨在探索山龙珠体外繁殖的最佳方案。在这方面,次氯酸钠(NaOCl)的混合物可以有效地对植物茎尖进行表面消毒,因为商用高乐氏和氯化汞(HgCl2)分别使用1.5% NaOCl和2.0 g/l HgCl2。茎尖外植体在含1.5 mg/l IBA的全ms培养基上培养。在芽增殖方面,含2 mg/l BA的ms培养基的芽数最高。对于离体生根,2.0 mg/l IBA的根数和根长最高。在温室条件下,生根后的植株在含有泥炭和沙子的花盆中以3:1的比例成活率为100%。
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Studies on Micro Propagation of Dracaena sanderiana Plants
The experimental was intended to find out the well protocol for in vitro propagation of Dracaena sanderiana. In this respect, plant shoot tips were effectively surface sterilized with a mixture of sodium hypochlorite (NaOCl) as commercial Clorox and mercuric chloride (HgCl2) were used at 1.5 % NaOCl and 2.0 g/l HgCl2. Shoot tip explants were cultured on full MS-medium contains 1.5 mg/l IBA to establishment. For shoot multiplication, MS-medium contains 2 mg/l BA produced the highest number of shoots. For in vitro rooting, 2.0 mg/l IBA gave the highest number of roots and root length. Plantlets after rooting exhibited 100% survival in pots containing peatmoss and sand at a ratio of 3:1 under greenhouse conditions.
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