热甲烷菌fervidus, sp. nov.,一种从冰岛温泉中分离出来的极端嗜热的新型甲烷菌

Karl O. Stetter , Michael Thomm, Josef Winter , Gertrud Wildgruber, Harald Huber, Wolfram Zillig , Davorin Jané-Covic , Helmut König, Peter Palm , Simon Wunderl
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引用次数: 178

摘要

描述了一种杆状极嗜热甲烷菌,生长在65至97℃之间,最佳温度约83℃,倍增时间为170 min。其DNA的gc含量为33 mol %。分离的细胞壁小囊含有假尿素。复杂的细胞包膜分为两层,每层厚度约为12nm;内层为假尿蛋白小囊,外层为蛋白包膜。RNA聚合酶的富集部分不能与热自养甲烷杆菌RNA聚合酶的抗血清发生反应,表明该分离物属于甲烷菌目甲烷热菌科。这种新生物被命名为热甲烷菌。
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Methanothermus fervidus, sp. nov., a novel extremely thermophilic methanogen isolated from an Icelandic hot spring

A rod-shaped extremely thermophilic methanogen is described, growing between 65 and 97 °C with an optimal temperature around 83 °C and a doubling time of 170 min. The GC-content of its DNA is 33 mol %. The isolated cell wall sacculus contains pseudomurein. The complex cell envelope exhibits two layers, each about 12 nm thick; the inner represents the pseudomurein sacculus and the outer a protein envelope.

An enriched fraction of RNA polymerase does not react with antiserum against RNA polymerase from Metbanobacterium thermoautotrophicum, indicating that the isolate belongs to a new family, the Methanothermaceae, within the order Methanobacteriales. The new organism is named Methanothermus fervidus.

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