Karl O. Stetter , Michael Thomm, Josef Winter , Gertrud Wildgruber, Harald Huber, Wolfram Zillig , Davorin Jané-Covic , Helmut König, Peter Palm , Simon Wunderl
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Methanothermus fervidus, sp. nov., a novel extremely thermophilic methanogen isolated from an Icelandic hot spring
A rod-shaped extremely thermophilic methanogen is described, growing between 65 and 97 °C with an optimal temperature around 83 °C and a doubling time of 170 min. The GC-content of its DNA is 33 mol %. The isolated cell wall sacculus contains pseudomurein. The complex cell envelope exhibits two layers, each about 12 nm thick; the inner represents the pseudomurein sacculus and the outer a protein envelope.
An enriched fraction of RNA polymerase does not react with antiserum against RNA polymerase from Metbanobacterium thermoautotrophicum, indicating that the isolate belongs to a new family, the Methanothermaceae, within the order Methanobacteriales. The new organism is named Methanothermus fervidus.