阿拉伯胶豆荚提取物的抑菌活性研究

Albert O. Fasogbon, J. Odewade, Ruth T. Omojoyegbe
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摘要

本研究旨在确定阿拉伯胶(Acacia nilotica)豆荚提取物对某些致病菌的抑菌潜力,这可能对健康有影响。分别用乙醇和蒸馏水提取相思豆荚粉。采用琼脂孔扩散法测定提取物对被试菌株的抑菌活性。提取物进一步进行定性植物化学筛选,以检测使用标准程序存在的次生代谢物。用标准方法测定了提取物的最低抑菌浓度和最低杀菌浓度。植物化学筛选在乙醇和水提取物中发现了某些代谢物(类固醇、皂苷、萜类、单宁、黄酮类、andand生物碱),除了类固醇,它们只存在于乙醇提取物中。结果表明,乙醇提取物在50mg/ml时对铜绿假单胞菌(22 mm)和伤寒沙门氏菌(15 mm)的抑制作用最强,而水提物在50mg/ml时对伤寒沙门氏菌(20 mm)和大肠杆菌(12 mm)的抑制作用最强。结果表明,拟南芥提取物具有潜在的抗菌作用。
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Antibacterial Potential of Pod Extracts of Gum Arabic Tree (Acacia nilotica)
This study was designed to determine the antibacterial potential of Gum Arabic Tree (Acacia nilotica) pod extracton some pathogenic bacteria which could have health implications. Powdered pods of Acacia nilotica wereextracted with ethanol and distilled water separately. The extracts were tested for antibacterial activities against thetest isolates using Agar Well Diffusion method. The extracts were further subjected to qualitative phytochemicalscreening to detect secondary metabolites present using standard procedures. The minimum inhibitory andminimum bactericidal concentrations of the extracts were also detected using standard procedures. Thephytochemical screening revealed certain metabolites (steroids, saponins, terpenoids, tannins, flavonoids, andalkaloids) in the ethanol and aqueous extracts, except for steroids, which were present only in ethanol extract. Theresult of the sensitivity test showed that ethanol pod extract produced the highest and least zone of inhibition againstPseudomonas aeruginosa (22 mm) and S. typhi (15 mm) respectively at 50mg/ml while the aqueous extract of thepod exhibited activity against S. typhi (20 mm) and E. coli (12 mm) at 50 mg/ml. This showed that the A. niloticapod extracts could serve as potential antibacterial agents against pathogenic bacteria.
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