香蕉(musa acuminata x balbisiana)假茎纤维制备的生物相容性和抗菌醋酸纤维素纳米纤维膜用于伤口愈合和组织工程

IF 1.3 4区 农林科学 Q2 MATERIALS SCIENCE, PAPER & WOOD Cellulose Chemistry and Technology Pub Date : 2023-09-29 DOI:10.35812/cellulosechemtechnol.2023.57.70
CARLO M. MACASPAG, JENNELI E. CAYA, JULIUS L. LEAÑO JR.
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引用次数: 0

摘要

静电纺丝纳米纤维膜在生物医学领域,特别是在伤口愈合和组织工程应用方面发挥着至关重要的作用。以香蕉假茎纤维为原料制备具有生物相容性和抗菌性能的醋酸纤维素静电纺丝纳米纤维膜。以碱处理过的香蕉假茎纤维为原料,通过酯化反应生产醋酸纤维素。将合成的醋酸纤维素和抗菌剂氯己定(CHX)溶解在2:1丙酮:N,N-二甲基乙酰胺溶剂中,然后电纺成纳米纤维膜。纳米纤维的FT-IR光谱证实了醋酸纤维素的存在以及CHX成功掺入纳米纤维中。扫描电镜成像表明,纳米纤维膜的纤维直径在200 ~ 300 nm之间。纳米纤维的MTT细胞毒实验和抗菌实验结果表明,以1.0 w/v的氯己定浓度制备的纳米纤维膜具有较强的抗菌活性(抑制区:大肠杆菌- 18.38 mm和金黄色葡萄球菌- 22.51 mm),对人肠上皮细胞HIEC-6具有较低的细胞毒性(细胞抑制率为13.07%,IC50为100 μg/mL)。结果表明,以香蕉假茎纤维为原料成功制备了具有生物相容性和抗菌性的纳米纤维膜,在伤口愈合和组织工程方面具有潜在的应用前景。
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BIOCOMPATIBLE AND ANTIMICROBIAL CELLULOSE ACETATE NANOFIBER MEMBRANE FROM BANANA (MUSA ACUMINATA X BALBISIANA) PSEUDOSTEM FIBERS FOR WOUND HEALING AND TISSUE ENGINEERING
Electrospun nanofiber membranes play a vital role in the biomedical field, especially for wound healing and tissue engineering applications. This study explored the development of biocompatible and antibacterial cellulose acetate electrospun nanofiber membranes prepared from banana pseudostem fibers. Cellulose rich dissolving pulp from alkali treated banana pseudostem fibers was subjected to esterification reaction to produce cellulose acetate. The synthesized cellulose acetate and chlorhexidine (CHX), an antimicrobial agent, were dissolved in 2:1 acetone:N,N-dimethylacetamide solvent and subsequently electrospun into a nanofiber membrane. FT-IR spectroscopy of the nanofiber confirmed the presence of cellulose acetate and the successful incorporation of CHX into the nanofibers. SEM imaging showed that the fiber diameter of the nanofiber membrane ranged from 200 nm to 300 nm. The MTT cytotoxicity assay and antimicrobial assay of nanofibers revealed that the nanofiber membrane with chlorhexidine concentration of 1.0 w/v was the optimum formulation as it achieved potent antimicrobial activity (zone of inhibition (ZOI): Escherichia coli – 18.38 mm and Staphylococcus aureus – 22.51 mm), while exhibiting low cytotoxicity to human intestinal epithelial cell line, HIEC-6 (percent cell inhibition: 13.07% and IC50: >100 μg/mL). The results indicated successful preparation of biocompatible and antimicrobial nanofiber membranes from banana pseudostem fiber with potential application in wound healing and tissue engineering.
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来源期刊
Cellulose Chemistry and Technology
Cellulose Chemistry and Technology 工程技术-材料科学:纸与木材
CiteScore
2.30
自引率
23.10%
发文量
81
审稿时长
7.3 months
期刊介绍: Cellulose Chemistry and Technology covers the study and exploitation of the industrial applications of carbohydrate polymers in areas such as food, textiles, paper, wood, adhesives, pharmaceuticals, oil field applications and industrial chemistry. Topics include: • studies of structure and properties • biological and industrial development • analytical methods • chemical and microbiological modifications • interactions with other materials
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