{"title":"同时使用新一代测序分析的Nested Multiplex PCR法，对赖氏菌的耐药性和型别进行新的同时鉴定法","authors":"Yasuhisa Iwao, Shuichi Mori, Manabu Ato, Noboru Nakata","doi":"10.5025/hansen.92.47","DOIUrl":null,"url":null,"abstract":"Mycobacterium leprae is the major etiologic agent of leprosy, and its genotype can be divided into four single nucleotide polymorphism (SNP) types and 16 subtypes. Drug resistance and genotype of M. leprae are typically determined using PCR and Sanger DNA sequencing, however, this requires a great deal of effort. In this review, we present a rapid method that we developed to identify drug resistance and SNP genotype of M. leprae directly from clinical specimens by combining nested multiplex PCR with next generation sequence analysis.","PeriodicalId":35918,"journal":{"name":"Japanese Journal of Leprosy","volume":"18 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"次世代シーケンシング解析を併用したNested Multiplex PCR法による、らい菌の薬剤耐性および型別の新規同時同定法\",\"authors\":\"Yasuhisa Iwao, Shuichi Mori, Manabu Ato, Noboru Nakata\",\"doi\":\"10.5025/hansen.92.47\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Mycobacterium leprae is the major etiologic agent of leprosy, and its genotype can be divided into four single nucleotide polymorphism (SNP) types and 16 subtypes. Drug resistance and genotype of M. leprae are typically determined using PCR and Sanger DNA sequencing, however, this requires a great deal of effort. In this review, we present a rapid method that we developed to identify drug resistance and SNP genotype of M. leprae directly from clinical specimens by combining nested multiplex PCR with next generation sequence analysis.\",\"PeriodicalId\":35918,\"journal\":{\"name\":\"Japanese Journal of Leprosy\",\"volume\":\"18 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Japanese Journal of Leprosy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5025/hansen.92.47\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese Journal of Leprosy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5025/hansen.92.47","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
Mycobacterium leprae is the major etiologic agent of leprosy, and its genotype can be divided into four single nucleotide polymorphism (SNP) types and 16 subtypes. Drug resistance and genotype of M. leprae are typically determined using PCR and Sanger DNA sequencing, however, this requires a great deal of effort. In this review, we present a rapid method that we developed to identify drug resistance and SNP genotype of M. leprae directly from clinical specimens by combining nested multiplex PCR with next generation sequence analysis.
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