{"title":"探讨抗氧化剂和抗氧化酶在洋葱种质中抗稻瘟菌的作用","authors":"S.S.A. Chauhan, Sabina Islam, Lakshman Prasad, Shrawan Singh, None R. Ellur, B.M. Rout, B.S. Tomar","doi":"10.53550/eec.2023.v29i03.008","DOIUrl":null,"url":null,"abstract":"The antioxidants and defense associated antioxidant enzymes associated with the entry of foreign bodies signal the first line of defense. In the present study, onion germplasm was analyzed for various biochemical parameters in response to inoculation Alternaria. porri for establishing role of antioxidant defense mechanism in onion for understanding their role in antioxidant-associated defense mechanism. The genotypes were categorized as resistant, moderately susceptible, susceptible and highly susceptible based on percent disease index (PDI). There were significant differences for all the parameters studied before and after inoculation. As compared to resistant genotypes, the susceptible and highly susceptible genotypes showed minimum total chlorophyll content at 3 weeks post-inoculation. The total phenol content in the leaf of resistant genotypes was high (246.4 mg GAE/100g FW) as compared to moderate susceptible (115.99 mg GAE/100g FW), susceptible (151.14 mg GAE/100g FW) and highly susceptible (146.60 mg GAE/100g FW) genotypes at 3 weeks post-inoculation (WPI). The antioxidant enzymes namely catalase, peroxidase and superoxide dismutase production increased upto 3 WPI. The activity of antioxidant enzymes (catalase, peroxidase and superoxide dismutase) was significantly high in resistant genotypes (9.80, 12.80 & 9.39 µmole/min/mg protein, respectively) as compared to moderately susceptible, susceptible and highly susceptible genotypes at 3 WPI. The study supports that antioxidant enzymes play a significant role in defense mechanism in conferring resistance against purple blotch and can be used as an effective bio-marker in resistance breeding against Alternaria porri .","PeriodicalId":11458,"journal":{"name":"Ecology, Environment and Conservation","volume":"2 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploring the role of elevated antioxidants and antioxidant enzymes in conferring resistance against Alternaria porri in onion germplasm\",\"authors\":\"S.S.A. Chauhan, Sabina Islam, Lakshman Prasad, Shrawan Singh, None R. Ellur, B.M. Rout, B.S. Tomar\",\"doi\":\"10.53550/eec.2023.v29i03.008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The antioxidants and defense associated antioxidant enzymes associated with the entry of foreign bodies signal the first line of defense. In the present study, onion germplasm was analyzed for various biochemical parameters in response to inoculation Alternaria. porri for establishing role of antioxidant defense mechanism in onion for understanding their role in antioxidant-associated defense mechanism. The genotypes were categorized as resistant, moderately susceptible, susceptible and highly susceptible based on percent disease index (PDI). There were significant differences for all the parameters studied before and after inoculation. As compared to resistant genotypes, the susceptible and highly susceptible genotypes showed minimum total chlorophyll content at 3 weeks post-inoculation. The total phenol content in the leaf of resistant genotypes was high (246.4 mg GAE/100g FW) as compared to moderate susceptible (115.99 mg GAE/100g FW), susceptible (151.14 mg GAE/100g FW) and highly susceptible (146.60 mg GAE/100g FW) genotypes at 3 weeks post-inoculation (WPI). The antioxidant enzymes namely catalase, peroxidase and superoxide dismutase production increased upto 3 WPI. The activity of antioxidant enzymes (catalase, peroxidase and superoxide dismutase) was significantly high in resistant genotypes (9.80, 12.80 & 9.39 µmole/min/mg protein, respectively) as compared to moderately susceptible, susceptible and highly susceptible genotypes at 3 WPI. The study supports that antioxidant enzymes play a significant role in defense mechanism in conferring resistance against purple blotch and can be used as an effective bio-marker in resistance breeding against Alternaria porri .\",\"PeriodicalId\":11458,\"journal\":{\"name\":\"Ecology, Environment and Conservation\",\"volume\":\"2 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ecology, Environment and Conservation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.53550/eec.2023.v29i03.008\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Environmental Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ecology, Environment and Conservation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.53550/eec.2023.v29i03.008","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Environmental Science","Score":null,"Total":0}
Exploring the role of elevated antioxidants and antioxidant enzymes in conferring resistance against Alternaria porri in onion germplasm
The antioxidants and defense associated antioxidant enzymes associated with the entry of foreign bodies signal the first line of defense. In the present study, onion germplasm was analyzed for various biochemical parameters in response to inoculation Alternaria. porri for establishing role of antioxidant defense mechanism in onion for understanding their role in antioxidant-associated defense mechanism. The genotypes were categorized as resistant, moderately susceptible, susceptible and highly susceptible based on percent disease index (PDI). There were significant differences for all the parameters studied before and after inoculation. As compared to resistant genotypes, the susceptible and highly susceptible genotypes showed minimum total chlorophyll content at 3 weeks post-inoculation. The total phenol content in the leaf of resistant genotypes was high (246.4 mg GAE/100g FW) as compared to moderate susceptible (115.99 mg GAE/100g FW), susceptible (151.14 mg GAE/100g FW) and highly susceptible (146.60 mg GAE/100g FW) genotypes at 3 weeks post-inoculation (WPI). The antioxidant enzymes namely catalase, peroxidase and superoxide dismutase production increased upto 3 WPI. The activity of antioxidant enzymes (catalase, peroxidase and superoxide dismutase) was significantly high in resistant genotypes (9.80, 12.80 & 9.39 µmole/min/mg protein, respectively) as compared to moderately susceptible, susceptible and highly susceptible genotypes at 3 WPI. The study supports that antioxidant enzymes play a significant role in defense mechanism in conferring resistance against purple blotch and can be used as an effective bio-marker in resistance breeding against Alternaria porri .
期刊介绍:
Published Quarterly Since 1995. Ecology, Environment and Conservation is published in March, June, September and December every year. ECOLOGY, ENVIRONMENT AND CONSERVATION is one of the leading International environmental journal. It is widely subsribed in India and abroad by Institutions and Individuals in education and research as well as by Industries, Govt. Departments and Research Institutes.