燕麦β-葡聚糖加蒙脱石粉对幼龄大鼠腹泻的治疗作用及机制

IF 4.6 Q1 CHEMISTRY, APPLIED Food Hydrocolloids for Health Pub Date : 2023-11-08 DOI:10.1016/j.fhfh.2023.100169
Shuyang Liu , Hamad Rafique , Liang Zou , Xinzhong Hu
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摘要

燕麦β-葡聚糖是一种影响肠道微生物群和维持能量稳态的益生元。燕麦β-葡聚糖有助于治疗腹泻和其他相关疾病,因为它可以调节肠道微生物群。研究燕麦β-葡聚糖联合蒙脱石粉对番泻叶提取物致幼年大鼠腹泻的治疗作用及机制。与腹泻模型组(DM)比较,联合给药,特别是中剂量蒙脱石粉加燕麦β-葡聚糖(M+G2)给药组,能有效减轻腹泻(P <0.05)和炎症指数,减轻结肠损伤,促进大鼠体重增加。在联合处理组中,门水平上厚壁菌门的相对丰度增加,而变形菌门和放线菌门的相对丰度降低。在属水平上,乳酸菌含量有所恢复,普雷沃氏菌和旁帕revotella等条件致病菌的比例有所下降。M+G2处理显著减少了幼龄大鼠腹泻,恢复了肠道菌群多样性,促进了短链脂肪酸(SCFAs)的产生。基于代谢组学,M+G2治疗抗腹泻作用的机制可能与调节葡萄糖和氨基酸代谢有关。通过磷酸戊糖和维生素B6途径改善了代谢微环境。代谢网络分析的核心代谢调节因子是l -天冬氨酸。总之,我们的研究结果表明,燕麦β-葡聚糖和蒙脱石粉的联合治疗可能通过调节炎症生物标志物、scfa和肠道微生物群,为治疗腹泻和相关疾病提供了有效的治疗策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Therapeutic effects and mechanism of oat β-glucan plus montmorillonite powder on diarrhea in young rats

Oat β-glucan is a prebiotic that affects intestinal microbiota and maintains energy homeostasis. Oat β-glucan helps treat diarrhea and other associated disorders, because it can regulate intestinal microbiota. We investigated the therapeutic effects and mechanism of oat β-glucan combined with montmorillonite powder on diarrhea induced by Senna leaf extract in young Sprague-Dawley rats. Compared with the diarrhea-induced model group (DM), the combined treatment, especially in the group treated with a medium dose of Montmorillonite powder plus oat β-glucan (M+G2), effectively reduced the diarrhea (P < 0.05) and inflammation indices, alleviated damage to the colon, and promoted weight gain in rats. In the combined treatment group, the relative abundance of Firmicutes increased at the phylum level, while the relative abundance of Proteobacteria and Actinobacteria decreased. At the genus level, the Lactobacillus content recovered, and the proportion of conditional pathogens, such as Prevotella and Paraprevotella decreased. M+G2 treatment significantly reduced diarrhea in young rats, restored intestinal microbiota diversity, and promoted the production of short-chain fatty acids (SCFAs). Based on metabolomics, the mechanism of the anti-diarrheal effect of M+G2 treatment may be related to the regulation of glucose and amino acid metabolism. The metabolic micro-environment was improved through the pentose phosphate and vitamin B6 pathways. The core metabolic regulator in metabolic network analysis was L-aspartic acid. Overall, our findings suggest that the combined treatment of oat β-glucan and montmorillonite powder may provide an effective therapeutic strategy for treating diarrhea and associated disorders by regulating the inflammatory biomarkers, SCFAs, and intestinal microbiota.

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