Isolation of lipase producing thermotolerant Bacillus subtilis TTP-06 from hot spring of Himachal Pradesh

Out of fifty-three isolates isolated from soil samples of Tattapani hot spring of Himachal Pradesh, India, nineteen were screened for lipase production. Isolate TTP-06 with lipase activity of 3.01±0.05 U/ml was characterized and optimized for different production conditions to give maximum production of lipase. Isolate TTP-06 was identified as Bacillus subtilis TTP-06. Maximum enzyme activity was observed when 0.75% (v/v) of 24 hrs old inoculum was used in production medium (pH 8.0) containing 2.0% (v/v) olive oil, 0.75% (w/v) peptone, 0.75% (w/v) glucose, 0.3% (w/v) NaCl, 0.05% (w/v) MgSO4 and incubated at 55ºC under shaking conditions (150 rpm) for 24 hrs.