Determination of diversity of actinomycete in forest soil subject to different blood groups; Classical approaches before metagenomic

Objective: Forensic microbiology is the developing field of forensic science. Forensic microbiologist can use trace evidence to match people to crime scenes, to investigate bioterrorism incidents, and to determine cause and time of death. In recent years, important studies on the environmental microbiota have been carried out. These studies can be associated with many legally valuable data as well as being related to the microbiota of environmental events. Actinobacteria are gram-positive micelle bacteria with enormous diversity, common in soil, and have high economic value. So far 47 families and 711 species of Actinobacteria were isolated and identified in variety of habitats. Increasing forensic studies in recent years show that microbial profiles can be used as evidence. Methods: In this study, actinobacteria were isolated from forest soil samples exposed to eight different blood groups for two weeks and control group. Genomic DNA isolation from colonies selected by considering