Optimal coordination between photosynthetic acclimation strategy and canopy architecture in two contrasting cucumber cultivars

Abstract Crop varieties differing in architectural characteristics (AC) vary in their intra-canopy light distribution. To optimize canopy photosynthesis, we hypothesize that varieties with contrasting AC possess different photosynthetic acclimation strategy (PAS) with respect to photosynthetic nitrogen (Np) partitioning. We firstly used in silico experiments to test this hypothesis and suggested a trade-off in Np partitioning between carboxylation and light harvesting to achieve optimal coordination between PAS, AC and growing light environment. Then, two cucumber (Cucumis sativus L.) cultivars, Aramon and SC-50, which were bred under greenhouse vertical single-stem and field creeping multi-branch canopy, were selected for studying their differences in AC and PAS using greenhouse and growth chamber experiments, respectively. In the greenhouse, more horizontal leaves of SC-50 resulted in steeper intra-canopy light gradient and a higher degree of self-shading, especially in the upper canopy layer. In growth chamber experiments, Aramon invested more leaf nitrogen into photosynthesis than SC-50, and the proportion (pNp) increased as light was reduced. In contrast, pNp of SC-50 did not respond to light but SC-50 partitioned its limited Np between carboxylation and light harvesting functions more effectively, showing a strategy particularly advantageous for canopies with a high degree of self-shading. This is further confirmed by additional in silico experiments showing that Np partitioning of SC-50 coped better with the impact of strong light competition caused by low light and by leaf clumping under high planting density. These findings provide a comprehensive perspective of genotypic variation in PAS, canopy architectures and their optimal coordination.