基于紫紫素和葫芦素的海藻酸盐超分子水凝胶[8]。宿主诱导的小泡介导的白细胞内吞作用

IF 9.7 4区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS VIEW Pub Date : 2023-10-13 DOI:10.1002/viw.20230058
Falguni Chandra, Maria Bykova, Vijo Poulose, Paltan Laha, Alina Aktanova, Olga Boeva, Margarita Barkovskaya, Ekaterina Pashkina, Na'il Saleh
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摘要

药物载体的细胞摄取到特定细胞的细胞质仍然具有挑战性,并且激发了非经典的超分子策略。在这里,我们选择了一个模型主-客体复合物,其中二氨基-紫素(1,1 ' -双(4 -氨基苯基)-[4,4 ' -联吡啶]- 1,1 ' -二氯化二钠[VG])荧光标签被葫芦[8]脲(CB8)吞噬,并与海藻酸多糖(海藻酸[ALG])共价连接,作为修饰的药物载体。当VG吸附在ALG表面时,利用傅里叶变换红外和核磁共振波谱方法首次证实了VG的包封性。固体光学测量(漫反射光谱、光致发光和时间分辨光致发光)显示,在650 nm左右发射材料,并且CB8增强了改性水凝胶的刚性。通过热重分析和差示扫描量热技术,确定了VG与CB8在海藻酸盐表面络合的摩尔组成为2:1,热稳定性。CB8诱导VGALG多糖的平均尺寸从485 nm急剧减小到165 nm,电荷从-19.8 mV转换到+14.4 mV。流式细胞术采用各种内吞途径抑制剂来跟踪不同血细胞类型的细胞摄取:人T细胞白血病1301和外周血单核细胞。值得注意的是,VG与CB8宿主在糖平台上的络合,通过小泡介导的内吞作用,在1.8 mg/mL的浓度下,通过体积减小、电荷由负向正转换和刚性诱导,显著增强了1301个细胞的内化(即从1%到99%)。这些观察结果揭示了对大环对药物传递的影响的更深刻的理解。
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Alginate supramolecular hydrogels based on viologen and cucurbit[8]uril: Host‐induced caveolae‐mediated endocytosis to white blood cancer cells
Abstract The cellular uptake of drug carriers to the cytosol of a specific cell remains challenging, and a non‐classical supramolecular strategy is motivated. Here, we select a model host–guest complex in which a diamino‐viologen (1,1′‐bis(4‐aminophenyl)‐[4,4′‐bipyridine]−1,1′‐diium dichloride [VG]) fluorescent tag was engulfed by cucurbit[8]uril (CB8) and covalently linked to alginate polysaccharides (alginic acid [ALG]) as the modified drug vehicle. When adsorbed on the ALG surface, the encapsulation of VG was first confirmed utilizing Fourier transform infrared and nuclear magnetic resonance spectroscopic methods. Solid optical measurements (diffuse reflectance spectroscopy, photoluminescence, and time‐resolved photoluminescence) revealed emissive materials at around 650 nm and that CB8 enhanced the rigidity of the modified hydrogel. The molar composition of 2:1 for the complexation of VG to CB8 on the alginate surface and the thermal stabilities were also confirmed using thermogravimetric analysis and differential scanning calorimetry techniques. CB8 induced a dramatic decrease in the average size of the VGALG polysaccharides from 485 to 165 nm and a turnover in their charge from –19.8 to +14.4 mV. Flow cytometry with inhibitors of various endocytosis pathways was employed to track the cellular uptake across different blood cell types: human T‐cell leukemia 1301 and peripheral blood mononuclear cells. Noticeably, complexation of VG with the CB8 host on top of the sugar platform dramatically enhanced the internalization into 1301 cells (viz. from 1% to 99%) at a concentration of 1.8 mg/mL via caveolae‐mediated endocytosis because of the size reduction, turnover in the charge from negative to positive, and rigidity induction. These observations reveal a more profound understanding of the macrocyclic effects on drug delivery.
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VIEW
VIEW Multiple-
CiteScore
12.60
自引率
2.30%
发文量
0
审稿时长
10 weeks
期刊介绍: View publishes scientific articles studying novel crucial contributions in the areas of Biomaterials and General Chemistry. View features original academic papers which go through peer review by experts in the given subject area.View encourages submissions from the research community where the priority will be on the originality and the practical impact of the reported research.
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