一些昆虫病原真菌对象鼻虫 Sitophilus granarius (L., 1758) (Coleoptera: Curculionidae) 的致病性

IF 0.9 4区 农林科学 Q3 ENTOMOLOGY Turkiye Entomoloji Dergisi-Turkish Journal of Entomology Pub Date : 2023-09-25 DOI:10.16970/entoted.1222114
Mahmut Alper ALTINOK, Memiş KESDEK, Şaban KORDALI, Ayşe USANMAZ BOZHÜYÜK, Hacer Handan ALTINOK
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引用次数: 0

摘要

本研究在25±1℃,75±5% RH,光照14h:黑暗10h的实验室条件下,测定了球孢白僵菌(Beauveria bassiana, ARSEF-4984)、土孢白僵菌(Isaria farinosa, ARSEF-3580)、烟孢白僵菌(Isaria fumosorsea, ARSEF-4501)、镰孢白僵菌(Lecanicillium muscarium, ARSEF-5128)、镰孢白僵菌(L. muscarium, ARSEF-972)、镰孢白僵菌(Lecanicillium lecanii, TR38/11)和镰孢白僵菌(L. muscarium, Ve6) 7株昆虫病原真菌分离株对粮象(Sitophilus granarius, L., 1758)(鞘翅目:Curculionidae)成虫的毒力。研究于2018年在atatatrk大学(Erzurum, t rkiye)进行。以两种不同的分生孢子浓度(1×105和1×107 ml-1)对成虫喷洒分离真菌。分别于治疗第2、4、6、8、10天观察死亡率。阳性对照为1株商业分离菌,阴性对照为+0.25% Tween 20无菌水。结果表明:昆虫病原真菌对黑麦成虫10 d的死亡率为1.01% ~ 98.9%;浓度越高,暴露时间越长,对成年个体的毒力越强。结果表明,在1×107 ml-1浓度下,实验第10天时,烟孢乳杆菌、蕈状乳杆菌(ARFES-5128)和lecanii乳杆菌的累积致死率分别为97.85%、94.62%和93.58%。这三种分离物被认为是很有前途的生物防治剂。
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Buğday biti Sitophilus granarius (L., 1758) (Coleoptera: Curculionidae) üzerinde bazı entomopatojen fungusların patojenisitesi
In the present study, the toxicities of seven entomopathogenic fungal isolates, Beauveria bassiana (ARSEF-4984), Isaria farinosa (ARSEF-3580), Isaria fumosorosea (ARSEF-4501), Lecanicillium muscarium (ARSEF-5128), L. muscarium (ARSEF-972), Lecanicillium lecanii (TR38/11) and L. muscarium (Ve6), were tested against the adults of granary weevil, Sitophilus granarius (L., 1758) (Coleoptera: Curculionidae), under laboratory conditions (25±1°C, 75±5% RH and 14h light:10h dark). Studies were conducted in Atatürk University (Erzurum, Türkiye), in 2018. Fungal isolates were sprayed to adults at two different conidial concentrations (1×105 and 1×107 ml-1). Mortality percentages were observed on the 2nd, 4th, 6th, 8th, and 10th days of treatment. A commercial isolate of L. muscarium were used as positive control and sterile water+0.25% Tween 20 used as negative control. The results demonstrated that the mortality rates of S. granarius adults treated with entomopathogenic fungi ranged from 1.01% to 98.9% across 10-day period. Higher concentration and longer exposure periods resulted in increasing virulence on the adult individuals. Among the strains tested, at 1×107 ml-1 concentration, I. fumosorosea, L. muscarium (ARFES-5128) and L. lecanii isolates displayed 97.85%, 94.62% and 93.58% cumulative mortalities respectively, on S. granarius adults by the 10th day of the experiment. These three isolates are regarded as highly promising biological control agents.
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来源期刊
CiteScore
1.60
自引率
12.50%
发文量
38
审稿时长
>12 weeks
期刊介绍: The Turkish Journal of Entomology is a quarterly journal which has been published by Entomological Society. Turkish Journal of Entomology publishes original research articles in the fields of entomology and agricultural zoology in English. Authors whose native language is not English should have their paper edited professionally prior to submission. Before preparing the typescript for submission, examine the format of manuscripts already published in Turkish Journal of Entomology. According to TJE rules, biological observations made in one location in only one-year, short notes in which the first record of a single species in Turkey is announced, and research which is older than five years will not be accepted for publication unless it forms part of a longitudinal study.
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