[毛癣菌抗原结构的研究]。

Polskie archiwum weterynaryjne Pub Date : 1989-01-01
K Kostro
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引用次数: 0

摘要

采用琼脂凝胶双扩散沉淀法和免疫电泳法研究马蹄草的抗原结构。制备兔沉淀血清时,发现用活抗原免疫效果最好。在马蹄草菌代谢抗原和水提物组中,发现10-11个血清+活性组分,而在蛋白质组分中仅发现L1组分。用天然血清和吸收血清进行交叉反应性对比研究,发现马蹄草和mentagrophytes的代谢抗原和水提取物有8个共同的组分,只有一个组分显示出物种分化特征。研究菌株中不规则地出现了标记为J和K的两个分数。在研究中使用的蛋白抗原中,只有一个部分(d)似乎是两个物种共同的,而其他三个部分是分开的。
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[Studies of the antigenic structure of Trichophyton equinum].

In the studies on antigenic structure of T. equinum double diffusion precipitation test in agar gel and immunoelectrophoresis were used. While preparing precipitation sera in rabbits it was manifested that the best results are achieved by immunization with live antigen. In the group of metabolic antigens and water extracts of the strains of T. equinum which were tested, 10-11 serologically+ active fractions were found whereas in protein component only L1 fraction. In comparative studies based on cross-reactivity method in which native and absorbed sera were used it was found that metabolic antigens and water extracts of T. equinum and T. mentagrophytes have 8 common fractions and only one fraction reveals species differentiation features. Two fractions, marked J and K, appeared in the studied strains of T. equinum irregularly. In protein antigen which was used in studies only one fraction (d) appeared to be common for both species, while three other fractions were separate.

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