新型镁铜杂化纳米材料治理番茄细菌性斑疹。

IF 4.4 2区 农林科学 Q1 PLANT SCIENCES Plant disease Pub Date : 2024-10-28 DOI:10.1094/PDIS-09-23-1776-RE
Manoj Choudhary, Ying-Yu Liao, Ziyang Huang, Jorge Pereira, Swadeshmukul Santra, Susannah Da Silva, Apekshya Parajuli, Joshua H Freeman, Jeffrey B Jones, Mathews L Paret
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引用次数: 0

摘要

番茄细菌性斑点病(BST)主要由佛罗里达州穿孔黄单胞菌(Xanthomonas perforans, Xp)引起,是炎热潮湿环境中最具破坏性的疾病之一。细菌对铜基杀菌剂和抗生素的耐药性使疾病管理极具挑战性。这就需要有其他办法来控制这种疾病。在这项研究中,我们使用了两种新型的铜镁混合纳米材料,即镁双包覆材料(Mg-Db)和镁铜(Mg-Cu)来处理BST。在体外实验中,Mg-Db和Mg-Cu浓度分别为500µg/ml、100µg/ml和200µg/ml的Mg-Db和Mg-Cu浓度分别为4 h后均未恢复活细胞,而完全抑制Mg-Db和Mg-Cu浓度需24 h。在活/死细胞培养法和荧光显微镜的活性测定中,500µg/ml的Mg-Cu和Mg-Db纳米材料在4小时内杀死了耐铜Xp细胞,而氢氧化铜(Kocide 3000)则没有。在温室中,Mg-Db和Mg-Cu浓度在100-500µg/ml时,与微米级商用杀菌剂Kocide 3000和种植者标准(氢氧化铜+代森锌)相比,显著降低了BST的严重程度(P < 0.05)。在实地研究中,Mg-Db和Mg-Cu纳米材料在两项野外试验中显著降低了疾病严重程度。与未处理的对照相比,500µg/ml Mg-Db在2020年秋季不影响产量的情况下降低了34%的BST严重程度。在田间试验中使用最高浓度(500µg/ml)的杂交纳米材料,与种植者的标准相比,可以减少90%的铜使用量。此外,在田间使用杂交纳米材料没有观察到植物毒性。这些结果表明,新型镁铜基杂化纳米材料具有控制耐铜细菌病原体的潜力。
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Novel Magnesium-Copper Hybrid Nanomaterials for Management of Bacterial Spot of Tomato.

Bacterial spot of tomato (BST), predominantly caused by Xanthomonas perforans (Xp) in Florida, is one of the most devastating diseases in hot, humid environments. Bacterial resistance to copper-based bactericides and antibiotics makes disease management extremely challenging. This necessitates alternative new solutions to manage the disease. In this study, we used two novel hybrid copper and magnesium nanomaterials, noted as magnesium double-coated (Mg-Db) and magnesium-copper (Mg-Cu), to manage BST. In in vitro experiments, no viable cells were recovered following 4 h of exposure to 500 μg/ml of both Mg-Db and Mg-Cu, while 100 and 200 μg/ml required 24 h of exposure for complete inhibition. In a viability assay using the live/dead cell straining method and epifluorescence microscopy, copper-tolerant Xp cells were killed within 4 h by both Mg-Cu and Mg-Db nanomaterials at 500 μg/ml but not by copper hydroxide (Kocide 3000). In the greenhouse, Mg-Db and Mg-Cu at 100 to 500 μg/ml significantly reduced BST severity compared with micron-sized commercial copper bactericide Kocide 3000 and the growers' standard (copper hydroxide + mancozeb) (P < 0.05). In field studies, Mg-Db and Mg-Cu nanomaterials significantly reduced disease severity in two out four field trials. Mg-Db at 500 μg/ml reduced BST severity by 34% compared with the nontreated control without affecting yield in fall, 2020. The use of hybrid nanomaterials at the highest concentrations (500 μg/ml) evaluated in the field experiments can reduce copper use by 90% compared with the growers' standard. In addition, there was no phytotoxicity observed with the use of hybrid nanomaterials in the field. These results suggest the potential of novel magnesium-copper-based hybrid nanomaterials to manage copper-tolerant bacterial pathogens.

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来源期刊
Plant disease
Plant disease 农林科学-植物科学
CiteScore
5.10
自引率
13.30%
发文量
1993
审稿时长
2 months
期刊介绍: Plant Disease is the leading international journal for rapid reporting of research on new, emerging, and established plant diseases. The journal publishes papers that describe basic and applied research focusing on practical aspects of disease diagnosis, development, and management.
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