围产期食源性二氧化钛暴露介导的生态失调使小鼠终生易患结肠炎。

IF 7.2 1区 医学 Q1 TOXICOLOGY Particle and Fibre Toxicology Pub Date : 2023-11-23 DOI:10.1186/s12989-023-00555-5
Caroline Carlé, Delphine Boucher, Luisa Morelli, Camille Larue, Ekaterina Ovtchinnikova, Louise Battut, Kawthar Boumessid, Melvin Airaud, Muriel Quaranta-Nicaise, Jean-Luc Ravanat, Gilles Dietrich, Sandrine Menard, Gérard Eberl, Nicolas Barnich, Emmanuel Mas, Marie Carriere, Ziad Al Nabhani, Frédérick Barreau
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引用次数: 0

摘要

背景:围产期暴露于二氧化钛(TiO2)作为食源性颗粒,可能会影响肠道屏障功能和在以后的生活中发生炎症性肠病(IBD)的易感性。在这里,我们研究围产期食源性二氧化钛暴露对肠黏膜功能的影响以及发生ibd相关结肠炎的易感性。将妊娠和哺乳期母鼠暴露于TiO2中直到幼崽断奶,并在出生后第30天(断奶)和成年后(50天)评估其后代的肠道微生物群和肠道屏障功能。通过测量结肠上皮细胞DNA中5-甲基-2'-脱氧胞嘧啶(5-Me-dC)水平的DNA甲基化谱研究表观遗传标记。采用葡聚糖硫酸钠(DSS)诱导的结肠炎模型监测IBD易感性。使用无菌小鼠来确定微生物转移是否影响dss诱导结肠炎的黏膜稳态和随后的恶化。结果:在怀孕和哺乳期小鼠中,食源性TiO2能够跨越宿主屏障,包括肠道、胎盘和乳腺,分别到达胚胎和幼崽。这一途径改变了母体及其子代胎儿和脾脏、肝脏的化学元素组成。我们发现,围产期早期暴露于TiO2会改变肠道菌群组成,增加肠上皮通透性,增强结肠细胞因子和肌球蛋白轻链激酶的表达。此外,围产期暴露于TiO2也会改变肠道干细胞的存活、生长和生成功能性上皮的能力。母体TiO2暴露增加了后代小鼠在以后的生活中发生严重dss诱导的结肠炎的易感性。最后,将二氧化钛诱导的微生物群失调转移到怀孕的无菌小鼠身上,会影响生命早期肠道黏膜屏障的稳态,并增加成年后代发生结肠炎的易感性。结论:我们的研究结果表明,围产期食源性TiO2的摄入对肠黏膜屏障的发育具有长期的负面影响,从而导致更高的结肠炎易感性。这表明环境因素在多大程度上影响了微生物与宿主的相互作用,并影响了长期的粘膜稳态。
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Perinatal foodborne titanium dioxide exposure-mediated dysbiosis predisposes mice to develop colitis through life.

Background: Perinatal exposure to titanium dioxide (TiO2), as a foodborne particle, may influence the intestinal barrier function and the susceptibility to develop inflammatory bowel disease (IBD) later in life. Here, we investigate the impact of perinatal foodborne TiO2 exposure on the intestinal mucosal function and the susceptibility to develop IBD-associated colitis. Pregnant and lactating mother mice were exposed to TiO2 until pups weaning and the gut microbiota and intestinal barrier function of their offspring was assessed at day 30 post-birth (weaning) and at adult age (50 days). Epigenetic marks was studied by DNA methylation profile measuring the level of 5-methyl-2'-deoxycytosine (5-Me-dC) in DNA from colic epithelial cells. The susceptibility to develop IBD has been monitored using dextran-sulfate sodium (DSS)-induced colitis model. Germ-free mice were used to define whether microbial transfer influence the mucosal homeostasis and subsequent exacerbation of DSS-induced colitis.

Results: In pregnant and lactating mice, foodborne TiO2 was able to translocate across the host barriers including gut, placenta and mammary gland to reach embryos and pups, respectively. This passage modified the chemical element composition of foetus, and spleen and liver of mothers and their offspring. We showed that perinatal exposure to TiO2 early in life alters the gut microbiota composition, increases the intestinal epithelial permeability and enhances the colonic cytokines and myosin light chain kinase expression. Moreover, perinatal exposure to TiO2 also modifies the abilities of intestinal stem cells to survive, grow and generate a functional epithelium. Maternal TiO2 exposure increases the susceptibility of offspring mice to develop severe DSS-induced colitis later in life. Finally, transfer of TiO2-induced microbiota dysbiosis to pregnant germ-free mice affects the homeostasis of the intestinal mucosal barrier early in life and confers an increased susceptibility to develop colitis in adult offspring.

Conclusions: Our findings indicate that foodborne TiO2 consumption during the perinatal period has negative long-lasting consequences on the development of the intestinal mucosal barrier toward higher colitis susceptibility. This demonstrates to which extent environmental factors influence the microbial-host interplay and impact the long-term mucosal homeostasis.

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来源期刊
CiteScore
15.90
自引率
4.00%
发文量
69
审稿时长
6 months
期刊介绍: Particle and Fibre Toxicology is an online journal that is open access and peer-reviewed. It covers a range of disciplines such as material science, biomaterials, and nanomedicine, focusing on the toxicological effects of particles and fibres. The journal serves as a platform for scientific debate and communication among toxicologists and scientists from different fields who work with particle and fibre materials. The main objective of the journal is to deepen our understanding of the physico-chemical properties of particles, their potential for human exposure, and the resulting biological effects. It also addresses regulatory issues related to particle exposure in workplaces and the general environment. Moreover, the journal recognizes that there are various situations where particles can pose a toxicological threat, such as the use of old materials in new applications or the introduction of new materials altogether. By encompassing all these disciplines, Particle and Fibre Toxicology provides a comprehensive source for research in this field.
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