{"title":"叶绿体基因组和核糖体DNA提供了高山通通虫分化和形态进化的见解","authors":"Ling-Jian Gui, Deng-Feng Xie, Chang Peng, Ting Ren, Li-Ying Yu, Song-Dong Zhou, Xing-Jin He","doi":"10.1111/jse.13028","DOIUrl":null,"url":null,"abstract":"<i>Tongoloa</i> is a herbaceous genus of East Asia Clade (Apiaceae) distributed in the alpine regions. The use of DNA fragments has not provided a well-resolved evolutionary history. For this research, we primarily collected samples from the type localities of <i>Tongoloa</i> and closely related taxa in the Hengduan Mountains. The chloroplast (cp) genomes and nuclear ribosomal (nr) DNA repeats of 27 taxa were assembled using genome skimming sequencing reads. We analyzed the characteristics of the <i>Tongoloa</i> cp genome, and found a remarkable expansion of the Inverted Repeats. Three genes (<i>ndhC, ndhJ</i>, and <i>petG</i>) related to photosynthesis appear to have undergone significant selective pressure. Through high-resolution phylogenetic analysis, the cpDNA provided compelling evidence supporting the inclusion of <i>Sinolimprichtia</i> as an early taxon within <i>Tongoloa</i>. However, the nrDNA suggested that <i>Tongoloa</i> and <i>Sinolimprichtia</i> belong to distinct branches. Morphological analysis showed that <i>Tongoloa</i> has broadly oval fruits with a cordate base, whereas the fruits of <i>Sinolimprichtia</i> are long-obovate with an obtuse base. The specific fruit morphology of <i>Sinolimprichtia</i> was found to be nested within <i>Tongoloa</i> in the cpDNA phylogenetic tree. Ancient introgression and chloroplast capture provide the most plausible explanation for the significant conflict between the nrDNA and cpDNA phylogenies. Our study highlights the potential impact of the complex evolutionary history of <i>Tongoloa</i> on the challenges encountered in previous taxonomic treatments.","PeriodicalId":17087,"journal":{"name":"Journal of Systematics and Evolution","volume":"83 ","pages":""},"PeriodicalIF":3.7000,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chloroplast genomes and ribosomal DNA provide insights into divergence and morphological evolution of alpine Tongoloa\",\"authors\":\"Ling-Jian Gui, Deng-Feng Xie, Chang Peng, Ting Ren, Li-Ying Yu, Song-Dong Zhou, Xing-Jin He\",\"doi\":\"10.1111/jse.13028\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<i>Tongoloa</i> is a herbaceous genus of East Asia Clade (Apiaceae) distributed in the alpine regions. The use of DNA fragments has not provided a well-resolved evolutionary history. For this research, we primarily collected samples from the type localities of <i>Tongoloa</i> and closely related taxa in the Hengduan Mountains. The chloroplast (cp) genomes and nuclear ribosomal (nr) DNA repeats of 27 taxa were assembled using genome skimming sequencing reads. We analyzed the characteristics of the <i>Tongoloa</i> cp genome, and found a remarkable expansion of the Inverted Repeats. Three genes (<i>ndhC, ndhJ</i>, and <i>petG</i>) related to photosynthesis appear to have undergone significant selective pressure. Through high-resolution phylogenetic analysis, the cpDNA provided compelling evidence supporting the inclusion of <i>Sinolimprichtia</i> as an early taxon within <i>Tongoloa</i>. However, the nrDNA suggested that <i>Tongoloa</i> and <i>Sinolimprichtia</i> belong to distinct branches. Morphological analysis showed that <i>Tongoloa</i> has broadly oval fruits with a cordate base, whereas the fruits of <i>Sinolimprichtia</i> are long-obovate with an obtuse base. The specific fruit morphology of <i>Sinolimprichtia</i> was found to be nested within <i>Tongoloa</i> in the cpDNA phylogenetic tree. Ancient introgression and chloroplast capture provide the most plausible explanation for the significant conflict between the nrDNA and cpDNA phylogenies. Our study highlights the potential impact of the complex evolutionary history of <i>Tongoloa</i> on the challenges encountered in previous taxonomic treatments.\",\"PeriodicalId\":17087,\"journal\":{\"name\":\"Journal of Systematics and Evolution\",\"volume\":\"83 \",\"pages\":\"\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2023-11-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Systematics and Evolution\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1111/jse.13028\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Systematics and Evolution","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/jse.13028","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Chloroplast genomes and ribosomal DNA provide insights into divergence and morphological evolution of alpine Tongoloa
Tongoloa is a herbaceous genus of East Asia Clade (Apiaceae) distributed in the alpine regions. The use of DNA fragments has not provided a well-resolved evolutionary history. For this research, we primarily collected samples from the type localities of Tongoloa and closely related taxa in the Hengduan Mountains. The chloroplast (cp) genomes and nuclear ribosomal (nr) DNA repeats of 27 taxa were assembled using genome skimming sequencing reads. We analyzed the characteristics of the Tongoloa cp genome, and found a remarkable expansion of the Inverted Repeats. Three genes (ndhC, ndhJ, and petG) related to photosynthesis appear to have undergone significant selective pressure. Through high-resolution phylogenetic analysis, the cpDNA provided compelling evidence supporting the inclusion of Sinolimprichtia as an early taxon within Tongoloa. However, the nrDNA suggested that Tongoloa and Sinolimprichtia belong to distinct branches. Morphological analysis showed that Tongoloa has broadly oval fruits with a cordate base, whereas the fruits of Sinolimprichtia are long-obovate with an obtuse base. The specific fruit morphology of Sinolimprichtia was found to be nested within Tongoloa in the cpDNA phylogenetic tree. Ancient introgression and chloroplast capture provide the most plausible explanation for the significant conflict between the nrDNA and cpDNA phylogenies. Our study highlights the potential impact of the complex evolutionary history of Tongoloa on the challenges encountered in previous taxonomic treatments.
期刊介绍:
Journal of Systematics and Evolution (JSE, since 2008; formerly Acta Phytotaxonomica Sinica) is a plant-based international journal newly dedicated to the description and understanding of the biological diversity. It covers: description of new taxa, monographic revision, phylogenetics, molecular evolution and genome evolution, evolutionary developmental biology, evolutionary ecology, population biology, conservation biology, biogeography, paleobiology, evolutionary theories, and related subjects.