Preparation and Evaluation of Nanoemulgel with Seed Oils for Skin Care

Skin is an external organ protecting the entire body from a complicated process that affects all living things. Vegetable oils give the epidermis a barrier that shields it and halts water loss. Polyunsaturated fatty acids, phenolic and flavonoid content present in grape seed oil (GSO), papaya seed oil (PSO), and flaxseed oil (FSO) are reported for their antioxidant and moisturizing effect when applied as a topical formulation for skin care. In the present work, the oils were screened for their antioxidant activity and skinwhitening properties and formulated as nanoemulgel. The oils were studied for their phenolic and flavonoid content and formulated as a nano emulgel using Tween 80 and Transcutol. Further, the formulation was evaluated for its physicochemical properties and stability. The oils' total phenolic and flavonoid content was determined using gallic acid and rutin trihydrate, respectively as standards. The % oleic acid content was determined by using HPTLC where PSO had the highest oleic acid content (54.04) as compared to GSO and FSO. All oils exhibited significant antioxidant activity and tyrosinase enzyme inhibition due to the phenolic components, flavonoids, and tocopherols. Based on the phytoactive present, nanoemulsion in different proportions (3% oil mix and 5% oil mix in a ratio of 1:1:1 and 1.5:1.5:2 for GSO: PSO: FSO) was formulated. Globule size and PDI of the optimized nanoemulsion batch were obtained as 181 nm and 0.292, respectively. Further optimization of formulation and its efficacy and dermatokinetics can be studied.