Synthesis and biological evaluation of amide derivatives of 1,2,4-thiadiazole-thiazole-pyridine as anticancer agents

A new series of amide derivatives (10a–j) were designed and synthesized and their structures were confirmed by analytical data. Further, all these derivatives (10a–j) were examined for in vitro antitumor activity against four human cancer cell lines like MCF-7 (breast), A549 (lung), Col-205 (colon) and A2780 (ovarian) by using of the MTT assay. All the derivatives (10a–j) were exhibited remarkable anticancer effects on four cell lines as compared with etoposide used as a positive control. The IC₅₀ values range of tested compounds from 0.11 ± 0.051 µM to 7.42 ± 5.89 µM; whereas, positive control showed IC₅₀ values ranges from 0.17 ± 0.034 µM to 3.34 ± 0.152 µM, respectively. Among these derivatives, four compounds 10a, 10b, 10c & 10d demonstrated more potent activities than the positive control.