驯鹿血清中抗穆勒激素的浓度取决于性激素水平

A. Krutikova, E. Nikitkina, G. V. Shiriaev, G. K. Peglivanian, A. A. Musidray, G. S. Nikitin
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摘要

驯鹿饲养是极北地区的主要农业部门。 从遗传和繁殖的角度来看,对驯鹿(Rangifer tarandus)的研究仍然很少,这阻碍了驯鹿业的进一步发展。目前正在积极开展驯鹿生产品质遗传学领域的研究,但对驯鹿生殖系统的形成和功能研究甚少。生殖过程的激素调节和生殖周期关键阶段激素状态的形成是动物生殖的一个 重要方面。驯鹿的生殖具有明显的季节性,研究生殖周期的激素调节是进一步利用辅助生殖技术的主要方面。这项工作致力于分析繁殖季节雌鹿体内抗穆勒氏管激素的水平。研究在布里亚特 "索约茨传统自然资源管理地区管理局 "MAU 的驯鹿养殖场进行(雌性,n=29)。抗缪勒氏管激素(AMH)是公认的雌性卵巢储备指标,研究了抗缪勒氏管激素浓度与雌性血清中性激素(孕酮、雌二醇、睾酮和黄体生成素)浓度的关系。激素水平是通过酶联免疫测定法测定的。结果显示,AMH 浓度大于 1 纳克/毫升的妇女组中性激素(睾酮、雌二醇、孕酮和黄体生成素)的含量高于 AMH 浓度为 1 纳克/毫升的妇女组。
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The concentration of anti-muller hormone in the blood serum of reindeer depends on the level of sex hormones
Reindeer  husbandry  is  the  leading branch of agriculture for the regions of the Far  North.  Reindeer  (Rangifer  tarandus) remains  poorly  studied  from  the  point  of view  of  genetics  and  reproduction,  which hinders the intensification of development of the industry. Research is being actively conduct in the field of genetics of the productive qualities of reindeer, but the formation and functioning  of  its  reproductive  system  has been little study. Hormonal regulation of the reproductive  process  and  the  formation  of hormonal status at key stages of the reproductive cycle is a significant aspect of animal reproduction. Reindeer are characterized by a pronounced seasonality of reproduction, and the study of hormonal regulation of the reproductive cycle is the main aspect of the further use of assisted reproductive technologies. The work is devoted to the analysis of the  level  of  anti-Mullerian  hormone  in  females during the breeding season. The studies were carried out on reindeer farms of the MAU “Directorate of the Territory of Traditional Natural Resources Management  of  Soyots”,  Buryatia,  (female, n=29). The dependence of the concentration of anti-Müllerian hormone (AMH), which is a generally accepted indicator of the female ovarian reserve, on the concentration of sex hormones  (progesterone,  estradiol,  testosterone and luteinizing hormone) in the blood serum of females was study. Hormone levels were  determined  by  enzyme  immunoassay. A higher content of sex hormones - testosterone,  estradiol,  progesterone  and  luteinizing  hormone  was  revealed  in  the  group  of women  with  an  AMH  concentration  of  >1 ng/ml than in the group of women with an AMH concentration of <1 ng/ml. The significance of the differences in progesterone was p<0.05,  and  in  estradiol – p<0.001.  A  low concentration  of  AMH  (<1  ng/ml),  characterizing a reduced ovarian reserve of female reindeer,  corresponds  to  a  reduced  concentration of sex hormones that support reproductive  function,  and,  conversely,  a  high concentration of AMH (>1 ng/ml), and, accordingly, a high ovarian reserve the reserve corresponds to an increased concentration of sex hormones - testosterone, estradiol, progesterone and luteinizing hormone.
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