从摩苏尔市腹泻儿童中分离出的隐孢子虫寄生虫的基因图谱

Hibba Alobide, Firas M. B. Alkhashab, Hiyam Altaee
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摘要

本研究于 2022 年 10 月初至 2023 年 3 月底期间进行,旨在使用显微镜和分子方法确定五岁以下儿童中副隐孢子虫的感染率。研究记录了 43 名(37.71%)感染儿童,诊断方法为改良蔡尔-尼尔森染色法(Modified Zeihl Neelsen Stain)和吉姆萨染色法(Gimsa stain)。另一方面,利用 PCR 对粪便样本进行的分子检测显示,在采集的 115 份粪便样本中,有 25 名(21.92%)儿童感染了这种寄生虫。对确诊感染隐孢子虫的标本进行了 DNA 测序,新分离的隐孢子虫首次在尼尼微省的 NCBI 登记,登记号为 757376。关键词:隐孢子虫病隐孢子虫病 基因图谱
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Genetic map of the isolated Cryptosporidium parasite from children with diarrheal in the city of Mosul
The current study was conducted between the beginning of October 2022 and the end of March 2023 to identify the prevalence of Cryptosporidium parivum infection among children under five years using microscopic and molecular methods. The study recorded 43(37.71%) infected children diagnosed using the Modified Zeihl Neelsen Stain and Gimsa stain. On the other hand, a molecular examination of stool samples using PCR showed that 25(21.92%) children out of 115 collected stool samples were infected with this parasite. DNA Sequencing was performed for specimens diagnosed with Cryptosporidium parasite, and the new isolates were registered for the first time in Nineveh governorate in NCBI under the accession number 757376. Keywords: Cryptosporidium disease, Genetic map.
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