{"title":"茴香精油纳米乳液对引起三七根腐病的镰孢菌有抑制作用","authors":"Hongyan Nie, Hongxin Liao, Jinrui Wen, Cuiqiong Ling, Liyan Zhang, Furong Xu, Xian Dong","doi":"10.1016/j.jgr.2023.12.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p><em>Fusarium oxysporum</em> (<em>F. oxysporum</em>) is the primary pathogenic fungus that causes <em>Panax notoginseng</em> (<em>P. notoginseng</em>) root rot disease. To control the disease, safe and efficient antifungal pesticides must currently be developed.</p></div><div><h3>Methods</h3><p>In this study, we prepared and characterized a nanoemulsion of <em>Foeniculum vulgare</em> essential oil (Ne-FvEO) using ultrasonic technology and evaluated its stability. Traditional <em>Foeniculum vulgare</em> essential oil (T-FvEO) was prepared simultaneously with 1/1000 Tween-80 and 20/1000 dimethyl sulfoxide (DMSO). The effects and inhibitory mechanism of Ne-FvEO and T-FvEO in <em>F. oxysporum</em> were investigated through combined transcriptome and metabolome analyses.</p></div><div><h3>Results</h3><p>Results showed that the minimum inhibitory concentration (MIC) of Ne-FvEO decreased from 3.65 mg/mL to 0.35 mg/mL, and its bioavailability increased by 10-fold. The results of gas chromatography/mass spectrometry (GC/MS) showed that T-FvEO did not contain a high content of estragole compared to <em>Foeniculum vulgare</em> essential oil (FvEO) and Ne-FvEO. Combined metabolome and transcriptome analysis showed that both emulsions inhibited the growth and development of <em>F. oxysporum</em> through the synthesis of the cell wall and cell membrane, energy metabolism, and genetic information of <em>F. oxysporum</em> mycelium. Ne-FvEO also inhibited the expression of 2-oxoglutarate dehydrogenase and isocitrate dehydrogenase and reduced the content of 2-oxoglutarate, which inhibited the germination of spores.</p></div><div><h3>Conclusion</h3><p>Our findings suggest that Ne-FvEO effectively inhibited the growth of <em>F. oxysporum</em> in <em>P. notoginseng</em> in vivo. The findings contribute to our comprehension of the antifungal mechanism of essential oils (EOs) and lay the groundwork for the creation of plant-derived antifungal medicines.</p></div>","PeriodicalId":16035,"journal":{"name":"Journal of Ginseng Research","volume":null,"pages":null},"PeriodicalIF":6.8000,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1226845323001835/pdfft?md5=1e8c6baf1449d2b3126c400b78176e3e&pid=1-s2.0-S1226845323001835-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Foeniculum vulgare essential oil nanoemulsion inhibits Fusarium oxysporum causing Panax notoginseng root-rot disease\",\"authors\":\"Hongyan Nie, Hongxin Liao, Jinrui Wen, Cuiqiong Ling, Liyan Zhang, Furong Xu, Xian Dong\",\"doi\":\"10.1016/j.jgr.2023.12.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p><em>Fusarium oxysporum</em> (<em>F. oxysporum</em>) is the primary pathogenic fungus that causes <em>Panax notoginseng</em> (<em>P. notoginseng</em>) root rot disease. To control the disease, safe and efficient antifungal pesticides must currently be developed.</p></div><div><h3>Methods</h3><p>In this study, we prepared and characterized a nanoemulsion of <em>Foeniculum vulgare</em> essential oil (Ne-FvEO) using ultrasonic technology and evaluated its stability. Traditional <em>Foeniculum vulgare</em> essential oil (T-FvEO) was prepared simultaneously with 1/1000 Tween-80 and 20/1000 dimethyl sulfoxide (DMSO). The effects and inhibitory mechanism of Ne-FvEO and T-FvEO in <em>F. oxysporum</em> were investigated through combined transcriptome and metabolome analyses.</p></div><div><h3>Results</h3><p>Results showed that the minimum inhibitory concentration (MIC) of Ne-FvEO decreased from 3.65 mg/mL to 0.35 mg/mL, and its bioavailability increased by 10-fold. The results of gas chromatography/mass spectrometry (GC/MS) showed that T-FvEO did not contain a high content of estragole compared to <em>Foeniculum vulgare</em> essential oil (FvEO) and Ne-FvEO. Combined metabolome and transcriptome analysis showed that both emulsions inhibited the growth and development of <em>F. oxysporum</em> through the synthesis of the cell wall and cell membrane, energy metabolism, and genetic information of <em>F. oxysporum</em> mycelium. Ne-FvEO also inhibited the expression of 2-oxoglutarate dehydrogenase and isocitrate dehydrogenase and reduced the content of 2-oxoglutarate, which inhibited the germination of spores.</p></div><div><h3>Conclusion</h3><p>Our findings suggest that Ne-FvEO effectively inhibited the growth of <em>F. oxysporum</em> in <em>P. notoginseng</em> in vivo. The findings contribute to our comprehension of the antifungal mechanism of essential oils (EOs) and lay the groundwork for the creation of plant-derived antifungal medicines.</p></div>\",\"PeriodicalId\":16035,\"journal\":{\"name\":\"Journal of Ginseng Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.8000,\"publicationDate\":\"2023-12-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1226845323001835/pdfft?md5=1e8c6baf1449d2b3126c400b78176e3e&pid=1-s2.0-S1226845323001835-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Ginseng Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1226845323001835\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Ginseng Research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1226845323001835","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
Fusarium oxysporum (F. oxysporum) is the primary pathogenic fungus that causes Panax notoginseng (P. notoginseng) root rot disease. To control the disease, safe and efficient antifungal pesticides must currently be developed.
Methods
In this study, we prepared and characterized a nanoemulsion of Foeniculum vulgare essential oil (Ne-FvEO) using ultrasonic technology and evaluated its stability. Traditional Foeniculum vulgare essential oil (T-FvEO) was prepared simultaneously with 1/1000 Tween-80 and 20/1000 dimethyl sulfoxide (DMSO). The effects and inhibitory mechanism of Ne-FvEO and T-FvEO in F. oxysporum were investigated through combined transcriptome and metabolome analyses.
Results
Results showed that the minimum inhibitory concentration (MIC) of Ne-FvEO decreased from 3.65 mg/mL to 0.35 mg/mL, and its bioavailability increased by 10-fold. The results of gas chromatography/mass spectrometry (GC/MS) showed that T-FvEO did not contain a high content of estragole compared to Foeniculum vulgare essential oil (FvEO) and Ne-FvEO. Combined metabolome and transcriptome analysis showed that both emulsions inhibited the growth and development of F. oxysporum through the synthesis of the cell wall and cell membrane, energy metabolism, and genetic information of F. oxysporum mycelium. Ne-FvEO also inhibited the expression of 2-oxoglutarate dehydrogenase and isocitrate dehydrogenase and reduced the content of 2-oxoglutarate, which inhibited the germination of spores.
Conclusion
Our findings suggest that Ne-FvEO effectively inhibited the growth of F. oxysporum in P. notoginseng in vivo. The findings contribute to our comprehension of the antifungal mechanism of essential oils (EOs) and lay the groundwork for the creation of plant-derived antifungal medicines.
期刊介绍:
Journal of Ginseng Research (JGR) is an official, open access journal of the Korean Society of Ginseng and is the only international journal publishing scholarly reports on ginseng research in the world. The journal is a bimonthly peer-reviewed publication featuring high-quality studies related to basic, pre-clinical, and clinical researches on ginseng to reflect recent progresses in ginseng research.
JGR publishes papers, either experimental or theoretical, that advance our understanding of ginseng science, including plant sciences, biology, chemistry, pharmacology, toxicology, pharmacokinetics, veterinary medicine, biochemistry, manufacture, and clinical study of ginseng since 1976. It also includes the new paradigm of integrative research, covering alternative medicinal approaches. Article types considered for publication include review articles, original research articles, and brief reports.
JGR helps researchers to understand mechanisms for traditional efficacy of ginseng and to put their clinical evidence together. It provides balanced information on basic science and clinical applications to researchers, manufacturers, practitioners, teachers, scholars, and medical doctors.