茴香精油纳米乳液对引起三七根腐病的镰孢菌有抑制作用

IF 6.8 2区 医学 Q1 CHEMISTRY, MEDICINAL Journal of Ginseng Research Pub Date : 2023-12-20 DOI:10.1016/j.jgr.2023.12.002
Hongyan Nie, Hongxin Liao, Jinrui Wen, Cuiqiong Ling, Liyan Zhang, Furong Xu, Xian Dong
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引用次数: 0

摘要

背景草孢霉(F. oxysporum)是引起三七根腐病的主要病原真菌。本研究利用超声波技术制备了茴香精油纳米乳液(Ne-FvEO),并对其稳定性进行了评估。传统茴香精油(T-FvEO)与 1/1000 吐温-80 和 20/1000 二甲基亚砜(DMSO)同时制备。结果结果表明,Ne-FvEO的最低抑菌浓度(MIC)从3.65 mg/mL降至0.35 mg/mL,生物利用度提高了10倍。气相色谱/质谱分析(GC/MS)结果表明,与茴香精油(FvEO)和Ne-FvEO相比,T-FvEO的雌甾醇含量并不高。代谢组和转录组的综合分析表明,这两种乳油都能通过细胞壁和细胞膜的合成、能量代谢以及草孢子菌丝体的遗传信息来抑制草孢子菌的生长和发育。Ne-FvEO 还能抑制 2-氧代戊二酸脱氢酶和异柠檬酸脱氢酶的表达,降低 2-氧代戊二酸的含量,从而抑制孢子的萌发。这些发现有助于我们理解精油(EO)的抗真菌机制,并为开发植物抗真菌药物奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Foeniculum vulgare essential oil nanoemulsion inhibits Fusarium oxysporum causing Panax notoginseng root-rot disease

Background

Fusarium oxysporum (F. oxysporum) is the primary pathogenic fungus that causes Panax notoginseng (P. notoginseng) root rot disease. To control the disease, safe and efficient antifungal pesticides must currently be developed.

Methods

In this study, we prepared and characterized a nanoemulsion of Foeniculum vulgare essential oil (Ne-FvEO) using ultrasonic technology and evaluated its stability. Traditional Foeniculum vulgare essential oil (T-FvEO) was prepared simultaneously with 1/1000 Tween-80 and 20/1000 dimethyl sulfoxide (DMSO). The effects and inhibitory mechanism of Ne-FvEO and T-FvEO in F. oxysporum were investigated through combined transcriptome and metabolome analyses.

Results

Results showed that the minimum inhibitory concentration (MIC) of Ne-FvEO decreased from 3.65 mg/mL to 0.35 mg/mL, and its bioavailability increased by 10-fold. The results of gas chromatography/mass spectrometry (GC/MS) showed that T-FvEO did not contain a high content of estragole compared to Foeniculum vulgare essential oil (FvEO) and Ne-FvEO. Combined metabolome and transcriptome analysis showed that both emulsions inhibited the growth and development of F. oxysporum through the synthesis of the cell wall and cell membrane, energy metabolism, and genetic information of F. oxysporum mycelium. Ne-FvEO also inhibited the expression of 2-oxoglutarate dehydrogenase and isocitrate dehydrogenase and reduced the content of 2-oxoglutarate, which inhibited the germination of spores.

Conclusion

Our findings suggest that Ne-FvEO effectively inhibited the growth of F. oxysporum in P. notoginseng in vivo. The findings contribute to our comprehension of the antifungal mechanism of essential oils (EOs) and lay the groundwork for the creation of plant-derived antifungal medicines.

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来源期刊
Journal of Ginseng Research
Journal of Ginseng Research CHEMISTRY, MEDICINAL-INTEGRATIVE & COMPLEMENTARY MEDICINE
CiteScore
11.40
自引率
9.50%
发文量
111
审稿时长
6-12 weeks
期刊介绍: Journal of Ginseng Research (JGR) is an official, open access journal of the Korean Society of Ginseng and is the only international journal publishing scholarly reports on ginseng research in the world. The journal is a bimonthly peer-reviewed publication featuring high-quality studies related to basic, pre-clinical, and clinical researches on ginseng to reflect recent progresses in ginseng research. JGR publishes papers, either experimental or theoretical, that advance our understanding of ginseng science, including plant sciences, biology, chemistry, pharmacology, toxicology, pharmacokinetics, veterinary medicine, biochemistry, manufacture, and clinical study of ginseng since 1976. It also includes the new paradigm of integrative research, covering alternative medicinal approaches. Article types considered for publication include review articles, original research articles, and brief reports. JGR helps researchers to understand mechanisms for traditional efficacy of ginseng and to put their clinical evidence together. It provides balanced information on basic science and clinical applications to researchers, manufacturers, practitioners, teachers, scholars, and medical doctors.
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