N. Pavan Kumar, Basavaraj D. Biradar, Basavaraj Bagewadi, N. G. Hanamaratti, Sumangala Bhat, Shekharappa, P. Nethra, Prashanth Kariyannanavar, N. M. Kavyashree
{"title":"与高粱（Sorghum bicolor (L. Moench)）A4（maldandi）雄性不育细胞质生育力恢复新性状相关的 SSR 标记的鉴定","authors":"N. Pavan Kumar, Basavaraj D. Biradar, Basavaraj Bagewadi, N. G. Hanamaratti, Sumangala Bhat, Shekharappa, P. Nethra, Prashanth Kariyannanavar, N. M. Kavyashree","doi":"10.1111/pbr.13155","DOIUrl":null,"url":null,"abstract":"Identification of markers associated with fertility restoration (<i>Rf</i>) genes is essential because they can streamline the breeding of new CMS lines and production of commercial hybrid seeds. Therefore, in the present study, F<sub>2</sub> populations (M31-2A × DSMR 8) was utilized to identify markers linked to <i>Rf</i> loci on <i>maldandi</i> (A<sub>4</sub>) cytoplasm through bulk segregant analysis (BSA). The F<sub>2</sub> population was analysed for seed set percentage. Chi-square (χ2) analysis showed that the fertility restoration trait followed expected digenic ratio. By BSA, simple sequence repeats (SSRs) markers, namely, <i>Xtxp 34</i> and <i>Xtxp 69</i> located on chromosome 3 and <i>SB 3956</i> and <i>Xtxp 312</i> located on chromosome 7, showed clear polymorphism between two groups of fertile and sterile bulks. The genomic region harbouring <i>Rf</i> locus on chromosome 3 (2.61 Mbp) predicted to encode five pentatricopeptide repeat (PPR) genes whereas, on chromosome 7, the gene <i>SORBI_3007G047400</i> predicted to encode MYB (myeloblastosis) domain containing proteins. These predicted genes could be the candidate for restoring fertility on A<sub>4</sub> cytoplasm. This finding will be fundamental in the production and rapid selection of novel restorer lines.","PeriodicalId":20228,"journal":{"name":"Plant Breeding","volume":"44 1","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification of SSR markers linked to new fertility restoration trait in sorghum (Sorghum bicolor (L.) Moench) for A4 (maldandi) male sterile cytoplasm\",\"authors\":\"N. Pavan Kumar, Basavaraj D. Biradar, Basavaraj Bagewadi, N. G. Hanamaratti, Sumangala Bhat, Shekharappa, P. Nethra, Prashanth Kariyannanavar, N. M. Kavyashree\",\"doi\":\"10.1111/pbr.13155\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Identification of markers associated with fertility restoration (<i>Rf</i>) genes is essential because they can streamline the breeding of new CMS lines and production of commercial hybrid seeds. Therefore, in the present study, F<sub>2</sub> populations (M31-2A × DSMR 8) was utilized to identify markers linked to <i>Rf</i> loci on <i>maldandi</i> (A<sub>4</sub>) cytoplasm through bulk segregant analysis (BSA). The F<sub>2</sub> population was analysed for seed set percentage. Chi-square (χ2) analysis showed that the fertility restoration trait followed expected digenic ratio. By BSA, simple sequence repeats (SSRs) markers, namely, <i>Xtxp 34</i> and <i>Xtxp 69</i> located on chromosome 3 and <i>SB 3956</i> and <i>Xtxp 312</i> located on chromosome 7, showed clear polymorphism between two groups of fertile and sterile bulks. The genomic region harbouring <i>Rf</i> locus on chromosome 3 (2.61 Mbp) predicted to encode five pentatricopeptide repeat (PPR) genes whereas, on chromosome 7, the gene <i>SORBI_3007G047400</i> predicted to encode MYB (myeloblastosis) domain containing proteins. These predicted genes could be the candidate for restoring fertility on A<sub>4</sub> cytoplasm. This finding will be fundamental in the production and rapid selection of novel restorer lines.\",\"PeriodicalId\":20228,\"journal\":{\"name\":\"Plant Breeding\",\"volume\":\"44 1\",\"pages\":\"\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2023-12-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Breeding\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1111/pbr.13155\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Breeding","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/pbr.13155","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
Identification of SSR markers linked to new fertility restoration trait in sorghum (Sorghum bicolor (L.) Moench) for A4 (maldandi) male sterile cytoplasm
Identification of markers associated with fertility restoration (Rf) genes is essential because they can streamline the breeding of new CMS lines and production of commercial hybrid seeds. Therefore, in the present study, F2 populations (M31-2A × DSMR 8) was utilized to identify markers linked to Rf loci on maldandi (A4) cytoplasm through bulk segregant analysis (BSA). The F2 population was analysed for seed set percentage. Chi-square (χ2) analysis showed that the fertility restoration trait followed expected digenic ratio. By BSA, simple sequence repeats (SSRs) markers, namely, Xtxp 34 and Xtxp 69 located on chromosome 3 and SB 3956 and Xtxp 312 located on chromosome 7, showed clear polymorphism between two groups of fertile and sterile bulks. The genomic region harbouring Rf locus on chromosome 3 (2.61 Mbp) predicted to encode five pentatricopeptide repeat (PPR) genes whereas, on chromosome 7, the gene SORBI_3007G047400 predicted to encode MYB (myeloblastosis) domain containing proteins. These predicted genes could be the candidate for restoring fertility on A4 cytoplasm. This finding will be fundamental in the production and rapid selection of novel restorer lines.
期刊介绍：
PLANT BREEDING publishes full-length original manuscripts and review articles on all aspects of plant improvement, breeding methodologies, and genetics to include qualitative and quantitative inheritance and genomics of major crop species. PLANT BREEDING provides readers with cutting-edge information on use of molecular techniques and genomics as they relate to improving gain from selection. Since its subject matter embraces all aspects of crop improvement, its content is sought after by both industry and academia. Fields of interest: Genetics of cultivated plants as well as research in practical plant breeding.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
