Somayeh Bahrami, Mohammad Mehdi Feizabadi, Nader Mosavari, Fattah Sotoodehnejad, Mohammad Eslampanah
{"title":"轻链 3 融合蛋白多表位在保护小鼠免受结核分枝杆菌感染方面的功效。","authors":"Somayeh Bahrami, Mohammad Mehdi Feizabadi, Nader Mosavari, Fattah Sotoodehnejad, Mohammad Eslampanah","doi":"10.30466/vrf.2023.1975747.3702","DOIUrl":null,"url":null,"abstract":"<p><p>The new strategy for vaccine development such as the fused protein multi-epitope capable of preventing the reactivation of latent tuberculosis infection (LTBi) can be an effective strategy for controlling tuberculosis (TB) worldwide. This study was conducted to evaluate the immunity of experimentally infected BALB/c mice with <i>Mycobacterium tuberculosis</i> after injection of DNA construct. Nineteen female BALB/c mice were divided into three groups and injected with 0.50 mL of <i>M. tuberculosis</i>. After 3 weeks, lung and spleen samples from the infected mice were examined. The protective effects of light chain 3-fused protein multi-epitope against TB were evaluated for post-exposure and therapeutic exposure. The lungs and spleens of the mice were aseptically removed after death for histopathology analysis. The bacterial colonies were counted, and the cells were stained after 3 weeks of incubation. No significant differences were observed between the post-exposure and therapeutic exposure groups. The pathological changes in the lung tissue of mice in these groups included an increase in the thickness of interalveolar septa, hyperemia, and intraparenchymal pulmonary hemorrhage centers (positive control), scattered hyperemic areas (negative control), and hyperemia in the interstitial tissue, scattered hyperemic areas in the lung parenchyma and lymphocytic infiltration centers (experimental group). Flow cytometry of the post-exposure and therapeutic exposure models showed insignificant changes in all three groups. It seems necessary to develop a post-exposure and therapeutic exposure vaccine strategy that focuses on LTBi to prevent the progression of the active disease. 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引用次数: 0
摘要
融合蛋白多表位能预防潜伏结核感染再活化(LTBi)等疫苗开发新战略可成为全球控制结核病(TB)的有效策略。本研究旨在评估实验性感染结核分枝杆菌的 BALB/c 小鼠注射 DNA 构建体后的免疫力。19 只雌性 BALB/c 小鼠被分成三组,分别注射 0.50 mL 结核分枝杆菌。3 周后,检查受感染小鼠的肺部和脾脏样本。评估了轻链 3-融合蛋白多表位在暴露后和治疗暴露中对结核病的保护作用。小鼠死亡后,无菌取出肺和脾脏进行组织病理学分析。对细菌菌落进行计数,并在培养 3 周后对细胞进行染色。暴露后组和治疗暴露组之间未发现明显差异。这些组别小鼠肺组织的病理变化包括肺泡间隔膜厚度增加、充血和肺实质内出血中心(阳性对照组)、散在充血区(阴性对照组),以及间质组织充血、肺实质散在充血区和淋巴细胞浸润中心(实验组)。暴露后和治疗暴露模型的流式细胞术显示,所有三组的变化都不明显。看来有必要制定以 LTBi 为重点的暴露后和治疗性暴露疫苗策略,以防止活动性疾病的发展。在这方面,应设计多表位疫苗来诱导细胞免疫和体液免疫。
Efficacy of light chain 3-fused protein multi epitope in protection of mice challenged with Mycobacterium tuberculosis.
The new strategy for vaccine development such as the fused protein multi-epitope capable of preventing the reactivation of latent tuberculosis infection (LTBi) can be an effective strategy for controlling tuberculosis (TB) worldwide. This study was conducted to evaluate the immunity of experimentally infected BALB/c mice with Mycobacterium tuberculosis after injection of DNA construct. Nineteen female BALB/c mice were divided into three groups and injected with 0.50 mL of M. tuberculosis. After 3 weeks, lung and spleen samples from the infected mice were examined. The protective effects of light chain 3-fused protein multi-epitope against TB were evaluated for post-exposure and therapeutic exposure. The lungs and spleens of the mice were aseptically removed after death for histopathology analysis. The bacterial colonies were counted, and the cells were stained after 3 weeks of incubation. No significant differences were observed between the post-exposure and therapeutic exposure groups. The pathological changes in the lung tissue of mice in these groups included an increase in the thickness of interalveolar septa, hyperemia, and intraparenchymal pulmonary hemorrhage centers (positive control), scattered hyperemic areas (negative control), and hyperemia in the interstitial tissue, scattered hyperemic areas in the lung parenchyma and lymphocytic infiltration centers (experimental group). Flow cytometry of the post-exposure and therapeutic exposure models showed insignificant changes in all three groups. It seems necessary to develop a post-exposure and therapeutic exposure vaccine strategy that focuses on LTBi to prevent the progression of the active disease. In this regard, multi-epitope vaccines should be designed to induce both cellular and humoral immunity.
期刊介绍:
Veterinary Research Forum (VRF) is a quarterly international journal committed to publish worldwide contributions on all aspects of veterinary science and medicine, including anatomy and histology, physiology and pharmacology, anatomic and clinical pathology, parasitology, microbiology, immunology and epidemiology, food hygiene, poultry science, fish and aquaculture, anesthesia and surgery, large and small animal internal medicine, large and small animal reproduction, biotechnology and diagnostic imaging of domestic, companion and farm animals.