Isabel C. Aksamit , Felipe Dorigão-Guimarães , Wulfila Gronenberg , R. Keating Godfrey
{"title":"白纹狮身蛾(Hyles lineata)的大脑大小在发育过程中不断扩大,其质量和细胞数量与苍蝇、蜜蜂和黄蜂相当。","authors":"Isabel C. Aksamit , Felipe Dorigão-Guimarães , Wulfila Gronenberg , R. Keating Godfrey","doi":"10.1016/j.asd.2023.101329","DOIUrl":null,"url":null,"abstract":"<div><p><span>Factors regulating larval growth and determinants of adult body size are described for several holometabolous insects, but less is known about brain<span> size scaling through development. Here we use the isotropic fractionation (“brain soup”) method to estimate the number of brain cells and cell density for the whitelined sphinx moth (Lepidoptera: </span></span><em>Hyles lineata</em><span>) from the first instar<span> through the adult stage. We measure mass and brain cell number and find that, during the larval stages, body mass shows an exponential relationship with head width, while the total number of brain cells increases asymptotically. Larval brain cell number increases by a factor of ten from nearly 8000 in the first instar to over 80,000 in the fifth instar. Brain cell number increases by another factor of 10 during metamorphosis, with the adult brain containing more than 900,000 cells. This is similar to increases during development in the vinegar fly (</span></span><span><em>Drosophila melanogaster</em></span><span>) and the black soldier fly (</span><em>Hermetia illucens</em><span>). The adult brain falls slightly below the brain-to-body allometry for wasps and bees but is comparable in the number of cells per unit brain mass, indicating a general conservation of brain cell density across these divergent lineages.</span></p></div>","PeriodicalId":55461,"journal":{"name":"Arthropod Structure & Development","volume":"78 ","pages":"Article 101329"},"PeriodicalIF":1.7000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Brain size scaling through development in the whitelined sphinx moth (Hyles lineata) shows mass and cell number comparable to flies, bees, and wasps\",\"authors\":\"Isabel C. Aksamit , Felipe Dorigão-Guimarães , Wulfila Gronenberg , R. Keating Godfrey\",\"doi\":\"10.1016/j.asd.2023.101329\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>Factors regulating larval growth and determinants of adult body size are described for several holometabolous insects, but less is known about brain<span> size scaling through development. Here we use the isotropic fractionation (“brain soup”) method to estimate the number of brain cells and cell density for the whitelined sphinx moth (Lepidoptera: </span></span><em>Hyles lineata</em><span>) from the first instar<span> through the adult stage. We measure mass and brain cell number and find that, during the larval stages, body mass shows an exponential relationship with head width, while the total number of brain cells increases asymptotically. Larval brain cell number increases by a factor of ten from nearly 8000 in the first instar to over 80,000 in the fifth instar. Brain cell number increases by another factor of 10 during metamorphosis, with the adult brain containing more than 900,000 cells. This is similar to increases during development in the vinegar fly (</span></span><span><em>Drosophila melanogaster</em></span><span>) and the black soldier fly (</span><em>Hermetia illucens</em><span>). The adult brain falls slightly below the brain-to-body allometry for wasps and bees but is comparable in the number of cells per unit brain mass, indicating a general conservation of brain cell density across these divergent lineages.</span></p></div>\",\"PeriodicalId\":55461,\"journal\":{\"name\":\"Arthropod Structure & Development\",\"volume\":\"78 \",\"pages\":\"Article 101329\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Arthropod Structure & Development\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1467803923000968\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Arthropod Structure & Development","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1467803923000968","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Brain size scaling through development in the whitelined sphinx moth (Hyles lineata) shows mass and cell number comparable to flies, bees, and wasps
Factors regulating larval growth and determinants of adult body size are described for several holometabolous insects, but less is known about brain size scaling through development. Here we use the isotropic fractionation (“brain soup”) method to estimate the number of brain cells and cell density for the whitelined sphinx moth (Lepidoptera: Hyles lineata) from the first instar through the adult stage. We measure mass and brain cell number and find that, during the larval stages, body mass shows an exponential relationship with head width, while the total number of brain cells increases asymptotically. Larval brain cell number increases by a factor of ten from nearly 8000 in the first instar to over 80,000 in the fifth instar. Brain cell number increases by another factor of 10 during metamorphosis, with the adult brain containing more than 900,000 cells. This is similar to increases during development in the vinegar fly (Drosophila melanogaster) and the black soldier fly (Hermetia illucens). The adult brain falls slightly below the brain-to-body allometry for wasps and bees but is comparable in the number of cells per unit brain mass, indicating a general conservation of brain cell density across these divergent lineages.
期刊介绍:
Arthropod Structure & Development is a Journal of Arthropod Structural Biology, Development, and Functional Morphology; it considers manuscripts that deal with micro- and neuroanatomy, development, biomechanics, organogenesis in particular under comparative and evolutionary aspects but not merely taxonomic papers. The aim of the journal is to publish papers in the areas of functional and comparative anatomy and development, with an emphasis on the role of cellular organization in organ function. The journal will also publish papers on organogenisis, embryonic and postembryonic development, and organ or tissue regeneration and repair. Manuscripts dealing with comparative and evolutionary aspects of microanatomy and development are encouraged.