Giorgio Gargari, Giacomo Mantegazza, Cesare Cremon, Valentina Taverniti, Alice Valenza, Maria Raffaella Barbaro, Giovanni Marasco, Robin Duncan, Walter Fiore, Roberto Ferrari, Valerio De Vitis, Giovanni Barbara, Simone Guglielmetti
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To this purpose, we performed a post-hoc analysis of samples collected during a multicenter, double-blind, parallel-group, placebo-controlled trial in which NC-IBS patients were randomized to receive at least 24 billion CFU LDG or placebo capsules <i>b.i.d</i>. for 12 weeks. The primary clinical endpoint was the composite response based on improved abdominal pain and fecal type. The fecal microbiome and serum markers of intestinal (PV1 and zonulin), liver, and kidney functions were investigated. We found that responders (R) in the probiotic arm (25%) differed from non-responders (NR) based on the abundance of 18 bacterial taxa, including the families <i>Coriobacteriaceae</i>, <i>Dorea</i> spp. and <i>Collinsella aerofaciens</i>, which were overrepresented in R patients. These taxa also distinguished R (but not NR) patients from healthy controls. Probiotic intervention significantly reduced the abundance of these bacteria in R, but not in NR. Analogous results emerged for <i>C. aerofaciens</i> from the analysis of data from a previous trial on IBS with the same probiotic. Finally, <i>C. aerofaciens</i> was positively correlated with the plasmalemmal vesicle associated protein-1 (PV-1) and the markers of liver function. In conclusion, LDG is effective on NC-IBS patients with NC-IBS with a greater abundance of potential pathobionts. Among these, <i>C. aerofaciens</i> has emerged as a potential predictor of probiotic efficacy.</p>","PeriodicalId":12909,"journal":{"name":"Gut Microbes","volume":"16 1","pages":"2298246"},"PeriodicalIF":12.2000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10773624/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>Collinsella aerofaciens</i> as a predictive marker of response to probiotic treatment in non-constipated irritable bowel syndrome.\",\"authors\":\"Giorgio Gargari, Giacomo Mantegazza, Cesare Cremon, Valentina Taverniti, Alice Valenza, Maria Raffaella Barbaro, Giovanni Marasco, Robin Duncan, Walter Fiore, Roberto Ferrari, Valerio De Vitis, Giovanni Barbara, Simone Guglielmetti\",\"doi\":\"10.1080/19490976.2023.2298246\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Probiotics are exploited for adjuvant treatment in IBS, but reliable guidance for selecting the appropriate probiotic to adopt for different forms of IBS is lacking. We aimed to identify markers for recognizing non-constipated (NC) IBS patients that may show significant clinical improvements upon treatment with the probiotic strain <i>Lacticaseibacillus paracasei</i> DG (LDG). To this purpose, we performed a post-hoc analysis of samples collected during a multicenter, double-blind, parallel-group, placebo-controlled trial in which NC-IBS patients were randomized to receive at least 24 billion CFU LDG or placebo capsules <i>b.i.d</i>. for 12 weeks. The primary clinical endpoint was the composite response based on improved abdominal pain and fecal type. The fecal microbiome and serum markers of intestinal (PV1 and zonulin), liver, and kidney functions were investigated. We found that responders (R) in the probiotic arm (25%) differed from non-responders (NR) based on the abundance of 18 bacterial taxa, including the families <i>Coriobacteriaceae</i>, <i>Dorea</i> spp. and <i>Collinsella aerofaciens</i>, which were overrepresented in R patients. These taxa also distinguished R (but not NR) patients from healthy controls. Probiotic intervention significantly reduced the abundance of these bacteria in R, but not in NR. Analogous results emerged for <i>C. aerofaciens</i> from the analysis of data from a previous trial on IBS with the same probiotic. Finally, <i>C. aerofaciens</i> was positively correlated with the plasmalemmal vesicle associated protein-1 (PV-1) and the markers of liver function. In conclusion, LDG is effective on NC-IBS patients with NC-IBS with a greater abundance of potential pathobionts. 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引用次数: 0
摘要
益生菌可用于肠易激综合征的辅助治疗,但目前还缺乏针对不同形式肠易激综合征选择合适益生菌的可靠指导。我们的目的是找出识别非便秘(NC)肠易激综合征患者的标志物,这些患者在接受益生菌菌株副乳酸杆菌 DG(LDG)治疗后,临床症状可能会明显改善。为此,我们对一项多中心、双盲、平行组、安慰剂对照试验中收集的样本进行了事后分析,在该试验中,NC 肠易激综合征患者被随机分配到至少 240 亿 CFU LDG 或安慰剂胶囊中,每日服用 12 周。主要临床终点是基于腹痛和粪便类型改善的综合反应。我们对粪便微生物群以及肠道(PV1 和 zonulin)、肝脏和肾脏功能的血清标志物进行了调查。我们发现,益生菌组中的应答者(R)(25%)与非应答者(NR)在 18 个细菌类群的丰度上存在差异,其中包括 Coriobacteriaceae、Dorea spp.和 Collinsella aerofaciens 科,这些类群在 R 患者中的比例较高。这些分类群还将 R 型(而非 NR 型)患者与健康对照组区分开来。益生菌干预大大降低了这些细菌在 R 患者中的含量,但在 NR 患者中却没有。通过对之前使用同一种益生菌进行的肠易激综合征试验数据进行分析,也得出了类似的结果。最后,铜绿假单胞菌与质膜囊泡相关蛋白-1(PV-1)和肝功能指标呈正相关。总之,LDG 对潜在致病菌较多的 NC-IBS 患者有效。其中,C. aerofaciens已成为益生菌疗效的潜在预测因子。
Collinsella aerofaciens as a predictive marker of response to probiotic treatment in non-constipated irritable bowel syndrome.
Probiotics are exploited for adjuvant treatment in IBS, but reliable guidance for selecting the appropriate probiotic to adopt for different forms of IBS is lacking. We aimed to identify markers for recognizing non-constipated (NC) IBS patients that may show significant clinical improvements upon treatment with the probiotic strain Lacticaseibacillus paracasei DG (LDG). To this purpose, we performed a post-hoc analysis of samples collected during a multicenter, double-blind, parallel-group, placebo-controlled trial in which NC-IBS patients were randomized to receive at least 24 billion CFU LDG or placebo capsules b.i.d. for 12 weeks. The primary clinical endpoint was the composite response based on improved abdominal pain and fecal type. The fecal microbiome and serum markers of intestinal (PV1 and zonulin), liver, and kidney functions were investigated. We found that responders (R) in the probiotic arm (25%) differed from non-responders (NR) based on the abundance of 18 bacterial taxa, including the families Coriobacteriaceae, Dorea spp. and Collinsella aerofaciens, which were overrepresented in R patients. These taxa also distinguished R (but not NR) patients from healthy controls. Probiotic intervention significantly reduced the abundance of these bacteria in R, but not in NR. Analogous results emerged for C. aerofaciens from the analysis of data from a previous trial on IBS with the same probiotic. Finally, C. aerofaciens was positively correlated with the plasmalemmal vesicle associated protein-1 (PV-1) and the markers of liver function. In conclusion, LDG is effective on NC-IBS patients with NC-IBS with a greater abundance of potential pathobionts. Among these, C. aerofaciens has emerged as a potential predictor of probiotic efficacy.
期刊介绍:
The intestinal microbiota plays a crucial role in human physiology, influencing various aspects of health and disease such as nutrition, obesity, brain function, allergic responses, immunity, inflammatory bowel disease, irritable bowel syndrome, cancer development, cardiac disease, liver disease, and more.
Gut Microbes serves as a platform for showcasing and discussing state-of-the-art research related to the microorganisms present in the intestine. The journal emphasizes mechanistic and cause-and-effect studies. Additionally, it has a counterpart, Gut Microbes Reports, which places a greater focus on emerging topics and comparative and incremental studies.