In vitro Evaluation of Antimicrobial Activity of Nigella sativa against Methicillin Resistant Staphylococcus aureus in Shendi Town, Sudan

Background: Augment of antibiotics resistance with development of new aggressive strains for the same disease is an international health problem. The ability of treating diseases is based on our ability to discover a new line of treatment with highly effectiveness and simple potentiality; so Herbal-Medicine is the line of choice. Traditional Arabic medicine point to the beneficial effect of Nigella sativa in curing a lot of diseases. The Prophetic-Sunna point out that Nigella sativa has highly therapeutic properties for various diseases. Prophet Mohammed quoted as saying “There is healing in Black seed for all diseases except death”. Methods: A cross-sectional community and hospital-based study, was done from 10 Jan-2023 to 31 March-2023 in Shendi Town, River Nile State. 95 nasal and skin swabs were collected from people and a variety of hospitals in shendi town, then cultured on Manitol Salt Agar (MSA) for selectivity of S.aureus to be isolated, bacterial identification done by colonial morphology, Gram`s stain and biochemical tests. The antimicrobial susceptibility of S.aureus to N. sativa was carried out using agar well diffusion method. Results: 48 pure culture of Methicillin resistant Staphylococcus aureus bacteria (MRSA) was isolated from 95 different clinical swabs specimens. Nigella sativa oil shows an effective inhibitory reactions against all MRSA bacterial isolates. All tested concentrations (50%, 25%, 13%) compared with Methicillin antibiotic inhibit 48(100%) of MRSA isolated bacteria. Conclusions: Our results show the highly effect of Nigella sativa against MRSA so can be derived as strongly effectiveness herbal antimicrobial agent against it.