从乳制品和母乳中分离和鉴定本地益生菌

Sumalata G, Shiva Prakash M
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摘要

益生菌是对人类有益的活微生物,许多研究已经证明它们作为安全的天然疗法具有广阔的前景。从本地资源中分离益生菌,可能会开发出一种新的方法来改良益生菌菌株,使其具有宝贵的医疗价值,造福人类。因此,本研究的目的是从当地的各种乳制品(即奶牛和水牛的生乳以及传统的自制凝乳)和母乳中分离出益生菌,并对其进行物种鉴定。研究人员无菌收集了约 80 份母乳(30 份)和乳制品(50 份)样本,并采用标准微生物学方法对其中的益生菌微生物群进行了筛查。挑出疑似益生菌的菌落进行形态学和生化鉴定,并通过分子方法和生物信息学的应用进一步确认。初步结果显示了几种不同的微生物菌群,即链球菌、葡萄球菌、乳酸杆菌、双球菌等。约十二(12)个分离物被进一步确认为属于乳酸杆菌属的不同物种。因此,乳制品和母乳被认为是益生微生物(尤其是乳酸杆菌属)的合适天然来源,据报道,这些益生微生物对人体有益。
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ISOLATION AND IDENTIFICATION OF INDIGENOUS PROBIOTICS FROM DAIRY PRODUCTS AND HUMAN BREAST MILK
Probiotics are live microbes that beneficially affect the human and many studies have proved their promising role as safe and natural therapeutics. The isolation of probiotics from indigenous sources may develop a new way to improved probiotic strains with precious medical relevance for human benefits. Hence the aim of the present study is to isolate probiotics from locally available various dairy products (Viz. raw milk of cow and buffalo and traditional homemade curds) and human breast milk and to identify them upto species level. A total of about 80 samples from human breast milk (n=30) and dairy products (n=50) were collected aseptically and screened for the probiotic microflora using standard microbiological methods. The colonies suspected for probiotics were picked up for morphological and biochemical identification which were further confirmed by molecular methods and application of bioinformatics. The preliminary results showed several different microflora viz. Streptococcus, Staphylococcus, Lactobacillus, Diplococcus, etc. About twelve (12) isolates were further confirmed to be of different species belonging to the Genus Lactobacillus. Therefore dairy products and human breast milk are considered as suitable natural sources for probiotic microorganisms especially Lactobacillus spp., which are reported to show beneficial effects in humans.
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