Elif Ayazoglu Demir, A. Menteşe, Hatice Küçük, Nihal Türkmen, S. Demir, Y. Aliyazicioglu
{"title":"N-乙酰半胱氨酸可改善 5-氟尿嘧啶诱发的大鼠卵巢损伤","authors":"Elif Ayazoglu Demir, A. Menteşe, Hatice Küçük, Nihal Türkmen, S. Demir, Y. Aliyazicioglu","doi":"10.33808/clinexphealthsci.1199615","DOIUrl":null,"url":null,"abstract":"Objective: Although 5-fluorouracil (5-FU) is one of the most commonly used chemotherapeutics worldwide, it has been shown that 5-FU administration can cause reproductive toxicity in recent years. N-acetylcysteine (NAC) is the precursor of glutathione, the most important endogenous antioxidant molecule and is known for its effective antioxidant and anti-inflammatory properties. Although NAC is one of the most studied antioxidant molecules, its curative effect against ovarian damage caused by 5-FU has not been demonstrated to date. It was therefore aimed to investigate whether NAC is therapeutic against 5-FU-induced ovotoxicity in this study for the first time. Methods: Rats were first exposed to a single dose of 5-FU (100 mg/kg) and then treated with NAC (10 and 20 mg/kg) for three days. The oxidative stress, inflammation and apoptosis markers in ovarian tissues were also determined using spectrophotometric methods. Ovarian tissues were also evaluated histologically. Results: It was revealed that the levels of oxidative stress, inflammation and apoptosis biomarkers in ovarian tissue increased by 5-FU administration (p","PeriodicalId":10192,"journal":{"name":"Clinical and Experimental Health Sciences","volume":null,"pages":null},"PeriodicalIF":0.3000,"publicationDate":"2023-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"N-Acetylcysteine Ameliorates 5-Fluorouracil‐Induced Ovarian Injury in Rats\",\"authors\":\"Elif Ayazoglu Demir, A. Menteşe, Hatice Küçük, Nihal Türkmen, S. Demir, Y. Aliyazicioglu\",\"doi\":\"10.33808/clinexphealthsci.1199615\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: Although 5-fluorouracil (5-FU) is one of the most commonly used chemotherapeutics worldwide, it has been shown that 5-FU administration can cause reproductive toxicity in recent years. N-acetylcysteine (NAC) is the precursor of glutathione, the most important endogenous antioxidant molecule and is known for its effective antioxidant and anti-inflammatory properties. Although NAC is one of the most studied antioxidant molecules, its curative effect against ovarian damage caused by 5-FU has not been demonstrated to date. It was therefore aimed to investigate whether NAC is therapeutic against 5-FU-induced ovotoxicity in this study for the first time. Methods: Rats were first exposed to a single dose of 5-FU (100 mg/kg) and then treated with NAC (10 and 20 mg/kg) for three days. The oxidative stress, inflammation and apoptosis markers in ovarian tissues were also determined using spectrophotometric methods. Ovarian tissues were also evaluated histologically. Results: It was revealed that the levels of oxidative stress, inflammation and apoptosis biomarkers in ovarian tissue increased by 5-FU administration (p\",\"PeriodicalId\":10192,\"journal\":{\"name\":\"Clinical and Experimental Health Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.3000,\"publicationDate\":\"2023-07-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical and Experimental Health Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33808/clinexphealthsci.1199615\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical and Experimental Health Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33808/clinexphealthsci.1199615","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
N-Acetylcysteine Ameliorates 5-Fluorouracil‐Induced Ovarian Injury in Rats
Objective: Although 5-fluorouracil (5-FU) is one of the most commonly used chemotherapeutics worldwide, it has been shown that 5-FU administration can cause reproductive toxicity in recent years. N-acetylcysteine (NAC) is the precursor of glutathione, the most important endogenous antioxidant molecule and is known for its effective antioxidant and anti-inflammatory properties. Although NAC is one of the most studied antioxidant molecules, its curative effect against ovarian damage caused by 5-FU has not been demonstrated to date. It was therefore aimed to investigate whether NAC is therapeutic against 5-FU-induced ovotoxicity in this study for the first time. Methods: Rats were first exposed to a single dose of 5-FU (100 mg/kg) and then treated with NAC (10 and 20 mg/kg) for three days. The oxidative stress, inflammation and apoptosis markers in ovarian tissues were also determined using spectrophotometric methods. Ovarian tissues were also evaluated histologically. Results: It was revealed that the levels of oxidative stress, inflammation and apoptosis biomarkers in ovarian tissue increased by 5-FU administration (p