{"title":"WRR4B 促进拟南芥对白粉病的广谱抗病性","authors":"Shuangshuang Mei, Yuxin Song, Zuer Zhang, Haitao Cui, Shuguo Hou, Weiguo Miao, Wei Rong","doi":"10.1111/mpp.13415","DOIUrl":null,"url":null,"abstract":"<i>Oidium heveae</i> HN1106, a powdery mildew (PM) that infects rubber trees, has been found to trigger disease resistance in <i>Arabidopsis thaliana</i> through ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)-, PHYTOALEXIN DEFICIENT 4 (PAD4)- and salicylic acid (SA)-mediated signalling pathways. In this study, a typical <i>TOLL-INTERLEUKIN 1 RECEPTOR, NUCLEOTIDE-BINDING, LEUCINE-RICH REPEAT</i> (<i>TIR-NB-LRR</i>)-encoding gene, <i>WHITE RUST RESISTANCE 4</i> (<i>WRR4B</i>), was identified to be required for the resistance against <i>O. heveae</i> in <i>Arabidopsis</i>. The expression of <i>WRR4B</i> was upregulated by <i>O. heveae</i> inoculation, and <i>WRR4B</i> positively regulated the expression of genes involved in SA biosynthesis, such as <i>EDS1</i>, <i>PAD4</i>, <i>ICS1</i> (<i>ISOCHORISMATE SYNTHASE 1</i>), <i>SARD1</i> (<i>SYSTEMIC-ACQUIRED RESISTANCE DEFICIENT 1</i>) and <i>CBP60g</i> (<i>CALMODULIN-BINDING PROTEIN 60 G</i>). Furthermore, WRR4B triggered self-amplification, suggesting that WRR4B mediated plant resistance through taking part in the SA-based positive feedback loop. In addition, WRR4B induced an <i>EDS1</i>-dependent hypersensitive response in <i>Nicotiana benthamiana</i> and contributed to disease resistance against three other PM species: <i>Podosphaera xanthii</i>, <i>Erysiphe quercicola</i> and <i>Erysiphe neolycopersici</i>, indicating that <i>WRR4B</i> is a broad-spectrum disease resistance gene against PMs.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"86 1","pages":""},"PeriodicalIF":4.8000,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"WRR4B contributes to a broad-spectrum disease resistance against powdery mildew in Arabidopsis\",\"authors\":\"Shuangshuang Mei, Yuxin Song, Zuer Zhang, Haitao Cui, Shuguo Hou, Weiguo Miao, Wei Rong\",\"doi\":\"10.1111/mpp.13415\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<i>Oidium heveae</i> HN1106, a powdery mildew (PM) that infects rubber trees, has been found to trigger disease resistance in <i>Arabidopsis thaliana</i> through ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)-, PHYTOALEXIN DEFICIENT 4 (PAD4)- and salicylic acid (SA)-mediated signalling pathways. In this study, a typical <i>TOLL-INTERLEUKIN 1 RECEPTOR, NUCLEOTIDE-BINDING, LEUCINE-RICH REPEAT</i> (<i>TIR-NB-LRR</i>)-encoding gene, <i>WHITE RUST RESISTANCE 4</i> (<i>WRR4B</i>), was identified to be required for the resistance against <i>O. heveae</i> in <i>Arabidopsis</i>. The expression of <i>WRR4B</i> was upregulated by <i>O. heveae</i> inoculation, and <i>WRR4B</i> positively regulated the expression of genes involved in SA biosynthesis, such as <i>EDS1</i>, <i>PAD4</i>, <i>ICS1</i> (<i>ISOCHORISMATE SYNTHASE 1</i>), <i>SARD1</i> (<i>SYSTEMIC-ACQUIRED RESISTANCE DEFICIENT 1</i>) and <i>CBP60g</i> (<i>CALMODULIN-BINDING PROTEIN 60 G</i>). Furthermore, WRR4B triggered self-amplification, suggesting that WRR4B mediated plant resistance through taking part in the SA-based positive feedback loop. In addition, WRR4B induced an <i>EDS1</i>-dependent hypersensitive response in <i>Nicotiana benthamiana</i> and contributed to disease resistance against three other PM species: <i>Podosphaera xanthii</i>, <i>Erysiphe quercicola</i> and <i>Erysiphe neolycopersici</i>, indicating that <i>WRR4B</i> is a broad-spectrum disease resistance gene against PMs.\",\"PeriodicalId\":18763,\"journal\":{\"name\":\"Molecular plant pathology\",\"volume\":\"86 1\",\"pages\":\"\"},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2024-01-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular plant pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1111/mpp.13415\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular plant pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/mpp.13415","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
WRR4B contributes to a broad-spectrum disease resistance against powdery mildew in Arabidopsis
Oidium heveae HN1106, a powdery mildew (PM) that infects rubber trees, has been found to trigger disease resistance in Arabidopsis thaliana through ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)-, PHYTOALEXIN DEFICIENT 4 (PAD4)- and salicylic acid (SA)-mediated signalling pathways. In this study, a typical TOLL-INTERLEUKIN 1 RECEPTOR, NUCLEOTIDE-BINDING, LEUCINE-RICH REPEAT (TIR-NB-LRR)-encoding gene, WHITE RUST RESISTANCE 4 (WRR4B), was identified to be required for the resistance against O. heveae in Arabidopsis. The expression of WRR4B was upregulated by O. heveae inoculation, and WRR4B positively regulated the expression of genes involved in SA biosynthesis, such as EDS1, PAD4, ICS1 (ISOCHORISMATE SYNTHASE 1), SARD1 (SYSTEMIC-ACQUIRED RESISTANCE DEFICIENT 1) and CBP60g (CALMODULIN-BINDING PROTEIN 60 G). Furthermore, WRR4B triggered self-amplification, suggesting that WRR4B mediated plant resistance through taking part in the SA-based positive feedback loop. In addition, WRR4B induced an EDS1-dependent hypersensitive response in Nicotiana benthamiana and contributed to disease resistance against three other PM species: Podosphaera xanthii, Erysiphe quercicola and Erysiphe neolycopersici, indicating that WRR4B is a broad-spectrum disease resistance gene against PMs.
期刊介绍:
Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.