Wanyue Li, Zeming Liu, Yuli Huang, Jie Zheng, Yang Yang, Yimeng Cao, Liwen Ding, Yuling Meng, Weixing Shan
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We confirmed the co-localization of Pi23014 and NbRBP3a within the nucleus, by using bimolecular fluorescence complementation. Reverse transcription-quantitative PCR assays showed that the expression of <i>NbRBP3a</i> was induced in <i>Nicotiana benthamiana</i> during <i>P. infestans</i> infection and the expression of marker genes for multiple defence pathways were significantly down-regulated in <i>NbRBP3</i>-silenced plants compared with <i>GFP</i>-silenced plants. <i>Agrobacterium tumefaciens</i>-mediated transient overexpression of <i>NbRBP3a</i> significantly enhanced plant resistance to <i>P. infestans</i>. Mutations in the N-terminus RNA recognition motif (RRM) of NbRBP3a abolished its interaction with Pi23014 and eliminated its capability to enhance plant resistance to leaf colonization by <i>P. infestans</i>. We further showed that silencing <i>NbRBP3</i> reduced photosystem II activity, reduced host photosynthetic efficiency, attenuated Pi23014-mediated suppression of cell death triggered by <i>P. infestans</i> pathogen-associated molecular pattern elicitor <i>INF1</i>, and suppressed plant immunity.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phytophthora infestans RXLR effector Pi23014 targets host RNA-binding protein NbRBP3a to suppress plant immunity\",\"authors\":\"Wanyue Li, Zeming Liu, Yuli Huang, Jie Zheng, Yang Yang, Yimeng Cao, Liwen Ding, Yuling Meng, Weixing Shan\",\"doi\":\"10.1111/mpp.13416\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<i>Phytophthora infestans</i> is a destructive oomycete that causes the late blight of potato and tomato worldwide. 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Reverse transcription-quantitative PCR assays showed that the expression of <i>NbRBP3a</i> was induced in <i>Nicotiana benthamiana</i> during <i>P. infestans</i> infection and the expression of marker genes for multiple defence pathways were significantly down-regulated in <i>NbRBP3</i>-silenced plants compared with <i>GFP</i>-silenced plants. <i>Agrobacterium tumefaciens</i>-mediated transient overexpression of <i>NbRBP3a</i> significantly enhanced plant resistance to <i>P. infestans</i>. Mutations in the N-terminus RNA recognition motif (RRM) of NbRBP3a abolished its interaction with Pi23014 and eliminated its capability to enhance plant resistance to leaf colonization by <i>P. infestans</i>. 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引用次数: 0
摘要
Phytophthora infestans 是一种毁灭性的卵菌,在全球范围内导致马铃薯和番茄的晚疫病。它分泌大量被称为效应子的小蛋白,以操纵宿主细胞成分并抑制植物免疫。确定这些效应子的靶标对于了解 P. infestans 的致病机理和宿主植物免疫至关重要。在这项研究中,我们发现 P. infestans 的毒力 RXLR 效应子 Pi23014 以宿主细胞核和叶绿体为靶标。通过液相色谱-串联质谱分析和共免疫沉淀分析,我们发现它与NbRBP3a(一种假定的富含甘氨酸的RNA结合蛋白)相互作用。通过双分子荧光互补,我们证实了 Pi23014 和 NbRBP3a 在细胞核内的共定位。逆转录-定量 PCR 分析表明,在 P. infestans 感染期间,NbRBP3a 在烟草中的表达被诱导,与 GFP 沉默的植株相比,NbRBP3 沉默的植株中多种防御途径的标记基因的表达显著下调。农杆菌介导的 NbRBP3a 瞬时过表达显著增强了植物对 P. infestans 的抗性。NbRBP3a的N端RNA识别基序(RRM)发生突变后,它与Pi23014的相互作用消失了,其增强植物对侵染病菌叶片定殖的抗性的能力也消失了。我们进一步发现,沉默 NbRBP3 会降低光系统 II 的活性,降低宿主的光合效率,减弱 Pi23014 介导的对 P. infestans 病原体相关分子模式诱导剂 INF1 引发的细胞死亡的抑制作用,并抑制植物的免疫力。
Phytophthora infestans RXLR effector Pi23014 targets host RNA-binding protein NbRBP3a to suppress plant immunity
Phytophthora infestans is a destructive oomycete that causes the late blight of potato and tomato worldwide. It secretes numerous small proteins called effectors in order to manipulate host cell components and suppress plant immunity. Identifying the targets of these effectors is crucial for understanding P. infestans pathogenesis and host plant immunity. In this study, we show that the virulence RXLR effector Pi23014 of P. infestans targets the host nucleus and chloroplasts. By using a liquid chromatogrpahy-tandem mass spectrometry assay and co-immunoprecipitation assasys, we show that it interacts with NbRBP3a, a putative glycine-rich RNA-binding protein. We confirmed the co-localization of Pi23014 and NbRBP3a within the nucleus, by using bimolecular fluorescence complementation. Reverse transcription-quantitative PCR assays showed that the expression of NbRBP3a was induced in Nicotiana benthamiana during P. infestans infection and the expression of marker genes for multiple defence pathways were significantly down-regulated in NbRBP3-silenced plants compared with GFP-silenced plants. Agrobacterium tumefaciens-mediated transient overexpression of NbRBP3a significantly enhanced plant resistance to P. infestans. Mutations in the N-terminus RNA recognition motif (RRM) of NbRBP3a abolished its interaction with Pi23014 and eliminated its capability to enhance plant resistance to leaf colonization by P. infestans. We further showed that silencing NbRBP3 reduced photosystem II activity, reduced host photosynthetic efficiency, attenuated Pi23014-mediated suppression of cell death triggered by P. infestans pathogen-associated molecular pattern elicitor INF1, and suppressed plant immunity.
期刊介绍:
Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.