Mariam Rusitashvili, Aza Kobakhidze, Vladimir Elisashvili
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引用次数: 0
摘要
本研究发现,在柑橘皮与 15 株白腐基枝菌浸没发酵过程中,木质素改性酶(LME)的种类和活性存在很大差异。其中,Trametes pubescens BCC153因同时产生漆酶、锰过氧化物酶(MnP)和木质素过氧化物酶(LiP)而脱颖而出。在培养四种曲霉的培养基中添加浓度为 1 mM 的 CuSO4 可成倍提高漆酶的产量。化学性质不同的木质纤维素生长基质和氮源对单个 LME 产量的不同影响已经确定。在含有柑橘皮和麦麸的培养基上培养 T. pubescens 时,可观察到其最大的漆酶活性,而在烟草残渣的浸没发酵中则检测到最高的 MnP 和 LiP 活性。蛋白胨和酪蛋白水解物似乎是 T. pubescens BCC153 产生漆酶和两种过氧化物酶的最佳氮源,而 KNO3 是产生所有酶的最差含氮化合物。
Activity of Lignin-Modifying Enzyme of Selected Medicinal Mushrooms in Submerged Fermentation of Lignocellulosic Materials
In the present study, wide diversity in the set and activity of lignin-modifying enzymes (LME) was revealed during submerged fermentation of mandarin peel with fifteen strains of white rot basidiomycetes. Among them, Trametes pubescens BCC153 was distinguished by the simultaneous production of laccase, manganese peroxidase (MnP), and lignin peroxidase (LiP). Supplementation of CuSO4 at a concentration of 1 mM in the media for the cultivation of four Trametes species manifold increased the production of laccase. The diverse effects of chemically different lignocellulosic growth substrates and nitrogen sources on the production of individual LME have been established. The maximum laccase activity of T. pubescens was observed when the fungus was cultivated on media containing mandarin peel and wheat bran, whereas the highest MnP and LiP activities were detected in the submerged fermentation of tobacco residue. Peptone and casein hydrolysate appeared to be the best sources of nitrogen for the production of laccase and both peroxidases by T. pubescens BCC153 whereas KNO3 was the worst nitrogen-containing compound for the production of all enzymes.
期刊介绍:
The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.