氟磺酰胺对黄铜质菌孢子萌发的影响

IF 1.5 4区 农林科学 Q2 ENTOMOLOGY Journal of Pesticide Science Pub Date : 2024-01-23 DOI:10.1584/jpestics.d23-031
Taito Sasaki, Ryohei Naito, Toshiaki Ohara, Kosei Sakane, Shuhei Tanaka, Kazunori Sasaki, Aoi Osato, Yuki Nomura, Shin-ichi Ito
{"title":"氟磺酰胺对黄铜质菌孢子萌发的影响","authors":"Taito Sasaki, Ryohei Naito, Toshiaki Ohara, Kosei Sakane, Shuhei Tanaka, Kazunori Sasaki, Aoi Osato, Yuki Nomura, Shin-ichi Ito","doi":"10.1584/jpestics.d23-031","DOIUrl":null,"url":null,"abstract":"</p><p>Flusulfamide inhibits germination of <i>Plasmodiophora brassicae</i> resting spores to suppress clubroot disease, but its mechanism of action on the germination of <i>P. brassicae</i> resting spores remains unclear. In this study, <i>P. brassicae</i> resting spores were treated with flusulfamide and visualized using transmission electron microscopy (TEM). The gene expression of <i>P. brassicae</i> resting spores was analyzed using RT-PCR, followed by immunoblotting analysis. TEM results revealed that flusulfamide suppressed the primary zoosporogenesis of <i>P. brassicae</i> resting spores during the early phase, and RT-PCR results revealed that flusulfamide affected the gene expression during the germination of the resting spores. Immunoblot and RT-qPCR analyses revealed that <i>PbCyp3</i>, an immunophilin (peptidyl-prolyl-isomerase) gene, was highly expressed, resulting in the unusual accumulation of PbCYP3 protein in <i>P. brassicae</i> resting spores immediately after treatment with flusulfamide. This suggests that flusulfamide may cause aberrant folding of proteins involved in primary zoosporogenesis, thereby inhibiting germination.</p>\n<p></p>\n<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D23-031/figure/advpub_D23-031.png\"/>\n<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of flusulfamide on spore germination of Plasmodiophora brassicae\",\"authors\":\"Taito Sasaki, Ryohei Naito, Toshiaki Ohara, Kosei Sakane, Shuhei Tanaka, Kazunori Sasaki, Aoi Osato, Yuki Nomura, Shin-ichi Ito\",\"doi\":\"10.1584/jpestics.d23-031\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"</p><p>Flusulfamide inhibits germination of <i>Plasmodiophora brassicae</i> resting spores to suppress clubroot disease, but its mechanism of action on the germination of <i>P. brassicae</i> resting spores remains unclear. In this study, <i>P. brassicae</i> resting spores were treated with flusulfamide and visualized using transmission electron microscopy (TEM). The gene expression of <i>P. brassicae</i> resting spores was analyzed using RT-PCR, followed by immunoblotting analysis. TEM results revealed that flusulfamide suppressed the primary zoosporogenesis of <i>P. brassicae</i> resting spores during the early phase, and RT-PCR results revealed that flusulfamide affected the gene expression during the germination of the resting spores. Immunoblot and RT-qPCR analyses revealed that <i>PbCyp3</i>, an immunophilin (peptidyl-prolyl-isomerase) gene, was highly expressed, resulting in the unusual accumulation of PbCYP3 protein in <i>P. brassicae</i> resting spores immediately after treatment with flusulfamide. This suggests that flusulfamide may cause aberrant folding of proteins involved in primary zoosporogenesis, thereby inhibiting germination.</p>\\n<p></p>\\n<img alt=\\\"\\\" src=\\\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D23-031/figure/advpub_D23-031.png\\\"/>\\n<span style=\\\"padding-left:5px;\\\">Fullsize Image</span>\",\"PeriodicalId\":16712,\"journal\":{\"name\":\"Journal of Pesticide Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-01-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Pesticide Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1584/jpestics.d23-031\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pesticide Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1584/jpestics.d23-031","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

氟磺酰胺可抑制黄刺浆孢属(Plasmodiophora brassicae)静止孢子的萌发,从而抑制球根病,但其对黄刺浆孢属静止孢子萌发的作用机制仍不清楚。在这项研究中,用氟磺酰胺处理了黄铜病菌的静止孢子,并用透射电子显微镜(TEM)进行了观察。使用 RT-PCR 分析了黄铜苣苔静止孢子的基因表达,然后进行了免疫印迹分析。透射电子显微镜结果显示,氟磺胺抑制了铜绿微囊藻静止孢子早期的初级动物孢子发生;RT-PCR结果显示,氟磺胺影响了静止孢子萌发过程中的基因表达。免疫印迹和 RT-qPCR 分析表明,PbCyp3(一种免疫嗜蛋白(肽基-脯氨酰-异构酶)基因)表达量很高,这导致在使用氟磺酰胺处理后,黄刺苣苔静止孢子中的 PbCYP3 蛋白会立即异常积累。这表明氟磺酰胺可能会导致参与初级动物孢子发生的蛋白质折叠异常,从而抑制发芽。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Effects of flusulfamide on spore germination of Plasmodiophora brassicae

Flusulfamide inhibits germination of Plasmodiophora brassicae resting spores to suppress clubroot disease, but its mechanism of action on the germination of P. brassicae resting spores remains unclear. In this study, P. brassicae resting spores were treated with flusulfamide and visualized using transmission electron microscopy (TEM). The gene expression of P. brassicae resting spores was analyzed using RT-PCR, followed by immunoblotting analysis. TEM results revealed that flusulfamide suppressed the primary zoosporogenesis of P. brassicae resting spores during the early phase, and RT-PCR results revealed that flusulfamide affected the gene expression during the germination of the resting spores. Immunoblot and RT-qPCR analyses revealed that PbCyp3, an immunophilin (peptidyl-prolyl-isomerase) gene, was highly expressed, resulting in the unusual accumulation of PbCYP3 protein in P. brassicae resting spores immediately after treatment with flusulfamide. This suggests that flusulfamide may cause aberrant folding of proteins involved in primary zoosporogenesis, thereby inhibiting germination.

Fullsize Image
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Pesticide Science
Journal of Pesticide Science 农林科学-昆虫学
CiteScore
4.30
自引率
4.20%
发文量
28
审稿时长
18-36 weeks
期刊介绍: The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.
期刊最新文献
Effect of pyriofenone on the infection processes and cytological features of Blumeria graminis on wheat leaves Synthesis and biological evaluation of burnettiene A derivatives enabling discovery of novel fungicide candidates. Enhanced disease resistance against Botrytis cinerea by strigolactone-mediated immune priming in Arabidopsis thaliana A reliable quantification of organophosphorus pesticides in brown rice samples for proficiency testing using Japanese official analytical method, QuEChERS, and modified QuEChERS combined with isotope dilution mass spectrometry Bacterial Pesticides: Mechanism of Action, Possibility of Food Contamination, and Residue Analysis Using MS
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1